相关实验视频

Updated: Jul 9, 2025

A Real-time Potency Assay for Chimeric Antigen Receptor T Cells Targeting Solid and Hematological Cancer Cells

A Real-time Potency Assay for Chimeric Antigen Receptor T Cells Targeting Solid and Hematological Cancer Cells

Published on: November 12, 2019

具有Ab-TCR和共刺激受体的双受体T细胞平台实现了针对AML的特异性和功效

Tao Dao¹, Guangyan Xiong², Sung Soo Mun¹

¹Molecular Pharmacology Program, Memorial Sloan Kettering Cancer Center, New York, NY.

|December 4, 2023

概括

一种新型的双向化学抗原受体T细胞 (CAR T) 疗法,AbTCR-CSR,通过向WT1和CD33抗原,增强特异性和降低毒性,对急性髓性白血病 (AML) 显示出有前途.

科学领域:

免疫学免疫学免疫学
在瘤学瘤学.
生物技术是生物技术.

背景情况:

化学抗原受体T细胞 (CAR T) 治疗在B细胞恶性瘤中取得了成功.
由于缺乏瘤特异性抗原和潜在的毒性,治疗固体瘤和其他血液癌症 (如急性髓性白血病 (AML)) 仍然存在挑战.

研究的目的:

开发一个双重向的CAR T细胞战略 (AbTCR-CSR) 针对AML.
设计针对威尔姆斯瘤1蛋白 (WT1) 和CD33抗原的T细胞,这些抗原在AML细胞上高度表达.

主要方法:

设计了一种新的T细胞平台,结合了向WT1的抗体-T细胞受体 (AbTCR) 和向CD33.3的仿真共刺激信号受体 (CSR).
使用TCR模仿单克隆抗体 (ESK2) 对抗WT1 RMF表位/HLA-A2复合体和单链可变片段对抗CD33.3.

主要成果:

改造的AbTCR-CSRT细胞对表达WT1和CD33.3的AML细胞表现出特定的细胞毒性.
这种方法节省了健康的造血细胞,包括正常的CD33+骨髓单细胞,表明减少了瘤外毒性.

结论:

AbTCR-CSR平台代表了一种潜在的战略,用于改善AML采用T细胞治疗的特异性和降低毒性.
这种双重准的方法可以提高急性髓性白血病患者的临床结果.

更多相关视频

A Three-dimensional Thymic Culture System to Generate Murine Induced Pluripotent Stem Cell-derived Tumor Antigen-specific Thymic Emigrants

A Three-dimensional Thymic Culture System to Generate Murine Induced Pluripotent Stem Cell-derived Tumor Antigen-specific Thymic Emigrants

Published on: August 9, 2019

Generating De Novo Antigen-specific Human T Cell Receptors by Retroviral Transduction of Centric Hemichain

Generating De Novo Antigen-specific Human T Cell Receptors by Retroviral Transduction of Centric Hemichain

Published on: October 25, 2016

相关概念视频

Diversity of Antigen Receptors

Diversity of Antigen Receptors

Antigen receptors are essential components of the immune system crucial in defending the body against foreign invaders. These receptors are present on the surface of B and T cells, enabling them to recognize antigens and mount an appropriate immune response.
Before encountering any antigen, lymphocytes express these receptors. On B cells, the antigen receptor is a membrane-bound antibody molecule called BCR; on T cells, it is a T cell receptor or TCR. B and T cell receptors are composed of two...

T Cell Activation and Clonal Selection

T Cell Activation and Clonal Selection

T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...

Special Features of Adaptive Immunity

Special Features of Adaptive Immunity

The adaptive immune system, a crucial component of the overall immune response, offers a highly specialized defense against pathogens. It involves specific cell types and features, enabling it to combat infections effectively and efficiently.
The primary cell types involved in adaptive immunity are T cells and B cells. Each type has a unique role in defending the body against pathogens. T cells are responsible for cell-mediated immunity. They identify and eliminate infected cells directly,...

Cytotoxic T Cells-mediated Immune Response

Cytotoxic T Cells-mediated Immune Response

Cytotoxic T cells are a vital component of the immune system. They have the remarkable ability to identify and target antigens on infected or abnormal cells. These antigens often originate from intracellular pathogens such as viruses or abnormal proteins cancer cells produce.
Immunological surveillance is the ability of immune cells to monitor and eliminate infected cells with intracellular pathogens, neoplastically transformed cells, and cells with non-self antigens. Cytotoxic T cells and NK...