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环境化学物质改变了成熟的人类白色脂肪细胞的细胞外脂质组

Paula Burkhardt¹, Susana Alejandra Palma-Duran², Astrud R R Tuck³

¹Department of Physiology and Pharmacology, Karolinska Institutet, Stockholm, Sweden; Institute of Physiology, University of Zurich, Zurich, Switzerland.

|December 4, 2023

概括

环境化学物质,称为代谢破坏化学物质 (MDCs),可以改变成熟的脂肪细胞功能. 脂质组分析揭示了细胞外脂质的敏感,化学特异性变化,表明早期代谢中断.

科学领域:

环境健康环境健康
内分泌学在内分泌学.
毒理学毒理学毒理学

背景情况:

代谢破坏性化学物质 (MDCs) 是与肥胖和2型糖尿病等代谢疾病相关的环境因素.
虽然MDCs对发育中的脂肪细胞的影响已知,但它们对成熟的白脂肪细胞和胰岛素耐药性的影响尚不清楚.
成熟的脂肪细胞是成年脂肪组织的关键组成部分,调节能量平衡.

研究的目的:

在实验室中研究六种MDC对成熟人体白脂肪细胞的影响.
确定MDC诱导的代谢干扰的敏感生物标志物和终点.
探索MDCs对脂肪细胞功能,基因表达和细胞外脂质谱的影响.

主要方法:

成熟的人类白色脂肪细胞,来源于介质干细胞,暴露于六种MDC (BPA,PFOA,TCS,ppDDE,TBT,TPP).
评估的终点包括脂肪细胞的葡萄糖消耗,脂质储存,脂肪细胞大小和基因表达 (GLUT1,勒丁,阿迪波内克丁).
在暴露3天后,对细胞培养基进行了细胞外脂质组分析.

主要成果:

高度的特里克洛桑 (TCS) 影响了脂质储存,脂肪细胞大小,葡萄糖消耗以及GLUT1,勒丁和阿迪波内克丁mRNA水平.
三酸 (TPP) 和罗西格利塔增加了阿迪波内克丁的表达.
脂质组分析揭示了大多数MDC的细胞外脂质的度依赖性变化,包括改变的lysophosphatidylcholines,glycerophospholipids,ceramides和脂肪酸.

结论:

环境MDCs可以诱导成熟脂肪细胞中代谢干扰的早期迹象.
细胞外脂质组分析是评估MDC不良影响的高度敏感的终点.
这些发现表明成熟脂肪细胞暴露于环境化学物质的潜在系统影响,并强调需要进行脂质学评估.

关键词:

内分泌干扰化学物质 (EDC) 是一种干扰内分泌的化学物质.能量的恒常状态 (Energy homeostasis) 是一种能量的恒常状态.脂质基因组分类型脂质基因组分类成熟的人类白色脂肪细胞代谢性疾病是一种代谢性疾病.破坏新陈代谢的化学物质 (MDC)

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