一种来自Enterococcus faecalis菌体的新型霍林和体外和体外应用
Yingying Xiang1, Suping Wang2, Hao Huang1
1Department of Stomatology, Yan'an Hospital Affiliated to Kunming Medical University, Kunming, 650031, China.
Microbial pathogenesis
|December 4, 2023
概括
菌体荷林pEF191对Enterococcus faecalis表现出强烈的抗菌活性,有效地去除生物膜并保护大鼠而不损害肠道微生物群. 这使得pEF191成为一种潜在的抗生素替代品.
科学领域:
- 微生物学 微生物学
- 细菌学 细菌学是一门学科.
- 分子生物学分子生物学
背景情况:
- 菌 (Enterococcus faecalis) 是一种耐受性强的病原体,导致持续性感染,特别是在根管治疗后.
- 在E. faecalis中抗生素耐药性迫切需要对新型治疗药物的探索.
- 来自菌体的蛋白质为对抗耐药细菌感染提供了一个有希望的途径.
研究的目的:
- 为了研究克隆的菌体holinpEF191对抗Enterococcus faecalis的抗菌潜力.
- 评估pEF191在消除E. faecalis生物膜方面的疗效及其在老鼠模型中的治疗效果.
- 评估pEF191对肠道微生物群组成的影响.
主要方法:
- 菌体荷林基因的克隆和表达,重组蛋白质的净化.
- 对pEF191.1.的抗菌活性,溶解概况和生物膜去除能力的分析.
- 在Sprague-Dawley大鼠体内治疗评估和16S rDNA分析肠道微生物群.
主要成果:
- 与父菌体相比,菌体holin pEF191表现出优越的抗菌作用和更广泛的溶解宿主范围.
- pEF191有效消除了E. faecalis生物膜,并在感染的老鼠中显示出显著的治疗性保护.
- pEF191没有对大鼠肠道微生物群多样性产生负面影响,并且改善了有益细菌的丰富性.
结论:
- 菌体荷林pEF191表现出强大的抗菌和抗生物膜活性对抗Enterococcus faecalis.
- pEF191在体内使用是安全的,对宿主动物或肠道微生物群没有不良影响.
- 霍林pEF191代表了对常规抗生素的有希望的替代品,用于管理E. faecalis感染.
相关概念视频
00:43Lysogenic Cycle of Bacteriophages
62.2K
In contrast to the lytic cycle, phages infecting bacteria via the lysogenic cycle do not immediately kill their host cell. Instead, they combine their genome with the host genome, allowing the bacteria to replicate the phage DNA along with the bacterial genome. The incorporated copy of the phage genome is called the prophage. Some prophages can re-activate and enter the lytic cycle. This often occurs in response to a perturbation, such as DNA damage, but can also transpire in the absence of...
62.2K
01:30Lytic Cycle of Bacteriophages
70.8K
Bacteriophages, also known as phages, are specialized viruses that infect bacteria. A key characteristic of phages is their distinctive “head-tail” morphology. A phage begins the infection process (i.e., lytic cycle) by attaching to the outside of a bacterial cell. Attachment is accomplished via proteins in the phage tail that bind to specific receptor proteins on the outer surface of the bacterium. The tail injects the phage’s DNA genome into the bacterial cytoplasm. In the...
70.8K