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开发一种二级方法,用于血中C4,C5和C2甲酸盐的分析

Josko Ivica¹, Faisal Adam², Lyse Wortel³

¹Department of Pathology and Molecular Medicine, McMaster University, Hamilton, Ontario, Canada; Hamilton Regional Laboratory Medicine Program, Hamilton Health Sciences, Hamilton, Ontario, Canada.

Clinical biochemistry

|December 4, 2023

概括

一种新的液态染色体质谱法 (LC-MS/MS) 方法有效地分离了甲,解决了同位体干扰. 这项技术通过准确量化关键的乙卡尼丁物种,改善了代谢先天性错误的诊断.

科学领域:

生物化学生化学
分析化学分析化学
代谢学代谢学代谢学

背景情况:

乙卡尼丁是代谢障碍的关键生物标志物.
传统的流量注入分析 (FIA) 缺乏对等离子干扰的解析.
液体染色学-质谱学 (LC-MS/MS) 提供了卓越的分离能力.

研究的目的:

开发和优化一种定性LC-MS/MS方法用于乙甲胺分析.
为了解决短链乙碳素 (2,4和5碳) 中的等离子干扰.
为了提高对代谢的先天性错误的诊断准确度.

主要方法:

使用丁醇HCl.Cl的乙卡尼丁衍生物化.
一种混合的同位素和梯度化LC-MS/MS方法.
多重反应监测 (MRM) 用于精确的离子检测.

主要成果:

成功地分离了丁和异丁卡尼丁.
成功分离了瓦莱瑞尔和异瓦莱瑞尔卡尼丁.
从干扰谷氨酸中区分出乙甲尼丁;在患者中确定异甲尼丁为主导的5碳乙甲尼丁.

结论:

建立了一种经过验证的质量LC-MS/MS方法,用于布틸衍生物的乙卡尼丁.
这种方法作为一个关键的二级诊断工具.
在医院网络内加强对代谢先天性错误的诊断和监测.

关键词:

乙卡尼提因是一种染色体学染色体学是一种染色学.代谢中的先天性错误质谱测量质量谱测量

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Development of Analytical Methods

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An analytical methodology can be divided into four sequential steps: technique, method, procedure, and protocol. A technique is a scientific principle that rationalizes a specific phenomenon through chemical measurements. Adapting a technique for analyzing a sample of interest is termed a method. The procedure outlines the directions for performing the analysis via an analytical method. The protocol is the detailed guidelines on the procedure, which should be strictly followed to obtain the...