线粒体ATP度在向缺乏葡萄糖的肝细胞注射葡萄糖后立即下降
Saki Tsuno1,2, Kazuki Harada1, Mina Horikoshi3
1Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, Tokyo, Japan.
FEBS open bio
|December 4, 2023
概括
肝细胞根据营养素调整新陈代谢. 这项研究揭示了线粒体腺三酸盐 (ATP) 在代谢转变期间的动态,显示其在调节蛋白质合成等细胞过程中的关键作用.
科学领域:
- 细胞的新陈代谢
- 肝细胞生物学 肝细胞生物学
- 生物化学 生物化学
背景情况:
- 肝细胞表现出代谢灵活性,适应营养的可用性.
- 在这些代谢切换期间的代谢物动态尚不清楚.
- 关键代谢物如乳酸盐,酸盐和腺三酸盐 (ATP) 都参与其中.
研究的目的:
- 在代谢切换期间可视化和理解肝细胞中关键代谢物的动态.
- 研究腺三酸盐 (ATP) 在调节营养恢复过程中的细胞过程中的作用.
主要方法:
- 使用初级培养肝细胞.
- 从葡萄糖剥夺恢复期间可视化代谢物动态.
- 测量了线粒体和细胞质腺三酸盐 (ATP) 的度.
主要成果:
- 在缺乏葡萄糖后服用葡萄糖后,观察到线粒体腺三酸盐 (ATP) 度的下降.
- 在细胞质腺三酸盐 (ATP) 度中发现了最小的变化.
- 线粒体腺三酸盐 (ATP) 的降低与蛋白质合成的增加有关,而不是糖原合成,尿素循环激活或活性氧物种的产生.
结论:
- 线粒体腺三酸盐 (ATP) 在介导肝细胞中的代谢开关中起着至关重要的作用.
- 这项研究强调了线粒体与细胞质腺三酸盐 (ATP) 在细胞调节中的不同作用.
- 研究结果提供了有关肝细胞代谢适应和能量管理的见解.
相关概念视频
ATP Energy Storage and Release
9.5K
ATP is a highly unstable molecule. Unless quickly used to perform work, ATP spontaneously dissociates into ADP and inorganic phosphate (Pi), and the free energy released during this process is lost as heat. The energy released by ATP hydrolysis is used to perform work inside the cell and depends on a strategy called energy coupling. Cells couple the exergonic reaction of ATP hydrolysis with endergonic reactions, allowing them to proceed.
One example of energy coupling using ATP involves a...
One example of energy coupling using ATP involves a...
9.5K
Outcomes of Glycolysis
99.5K
Nearly all the energy used by cells comes from the bonds that make up complex organic compounds. These organic compounds are broken down into simpler molecules, such as glucose. As a result, cells extract energy from glucose over many chemical reactions—a process called cellular respiration.
Cellular respiration can occur aerobically (with oxygen) or anaerobically (without oxygen). In the presence of oxygen, cellular respiration starts with glycolysis and continues with pyruvate...
Cellular respiration can occur aerobically (with oxygen) or anaerobically (without oxygen). In the presence of oxygen, cellular respiration starts with glycolysis and continues with pyruvate...
99.5K
ATP Yield
69.5K
Cellular respiration produces 30 - 32 ATP per glucose molecule. Although most of the ATP results from oxidative phosphorylation and the electron transport chain (ETC), 4 ATP are gained beforehand (2 from glycolysis and 2 from the citric acid cycle).
The ETC is embedded in the inner mitochondrial membrane and is comprised of four main protein complexes and an ATP synthase. NADH and FADH2 pass electrons to these complexes, which pump protons into the intermembrane space. This distribution of...
The ETC is embedded in the inner mitochondrial membrane and is comprised of four main protein complexes and an ATP synthase. NADH and FADH2 pass electrons to these complexes, which pump protons into the intermembrane space. This distribution of...
69.5K


