相关实验视频

Updated: Jul 9, 2025

Enteric Bacterial Invasion Of Intestinal Epithelial Cells In Vitro Is Dramatically Enhanced Using a Vertical Diffusion Chamber Model

Enteric Bacterial Invasion Of Intestinal Epithelial Cells In Vitro Is Dramatically Enhanced Using a Vertical Diffusion Chamber Model

Published on: October 22, 2013

食物和水中传播的阿科巴克特菌种在Caco-2中表现出高毒性活性

Itsaso Baztarrika¹, Adrián Salazar-Sánchez¹, Lorena Laorden²

¹MikroIker Research Group, Department of Immunology, Microbiology and Parasitology, Faculty of Pharmacy, University of the Basque Country UPV/EHU, Paseo de la Universidad 7, 01006, Vitoria-Gasteiz, Spain.

Food microbiology

|December 4, 2023

概括

来自食物和水中的阿科巴克特菌种粘附并入侵人类细胞,污水分离物显示出更高的毒性. 像cadF和heca这样的特定基因与这种粘附能力有关.

科学领域:

微生物学微生物学
食品安全食品安全
传染性疾病传染性疾病

背景情况:

目前尚不完全了解阿科巴克特菌感染的机制.
影响Arcobacter引起疾病的能力的毒性因素需要进一步调查.

研究的目的:

评估Arcobacter分离物在Caco-2细胞中的粘附和入侵能力.
将这些能力与特定的遗传标记 (cadF, cj1349, ciaB, hecA) 相关联.
为了比较不同Arcobacter物种和来源之间的毒性.

主要方法:

测试了65种通过食物和水传播的Arcobacter分离物 (至少六种) 进行Caco-2细胞粘附和入侵.
选隔离物以检测有毒性标记基因的存在:cadF, cj1349, ciaB和 hecA.
基于物种和来源 (例如,污水) 的毒性和基因含量的统计分析差异.

主要成果:

所有测试的阿科巴克特菌分离物都附着并侵入了Caco-2细胞,具有至少两种毒性标记物.
阿尔科巴克特·布茨莱里 (Arcobacter butzleri) 的毒性比阿尔科巴克特·克里亚罗菲勒斯 (Arcobacter cryaerophilus) 的毒性更高.
阿尔科巴克特兰蒂埃里,阿尔科巴克特斯基克罗维和阿尔科巴克特维托里恩斯表现出优越的粘附和入侵能力.
与其他来源相比,污水分离体显示出明显更高的粘附性,入侵性和基因含量.
在cadF和heca基因与高粘附能力之间发现了一种新的关联.

结论:

在食物和水中常见的阿科巴克特菌种类具有致病潜力.
阿尔科巴克特·布茨莱里比阿尔科巴克特·克里亚罗菲勒斯更具毒性,更少见的物种如A. lanthieri和A. vitoriensis也具有高度的毒性.
污水是潜在更具毒性的Arcobacter菌株的重要储存库,这突出了其在公共卫生监测中的重要性.

关键词:

这种细菌是Arcobacter spp.这是一种Caco-2感染.食品食品食品食品这是一个PCRPCR.河水河水河水河水污水污水处理污水污水处理

更多相关视频

A Co-culture Method to Investigate the Crosstalk Between X-ray Irradiated Caco-2 Cells and PBMC

A Co-culture Method to Investigate the Crosstalk Between X-ray Irradiated Caco-2 Cells and PBMC

Published on: January 30, 2018

Single Cell Measurements of Vacuolar Rupture Caused by Intracellular Pathogens

Single Cell Measurements of Vacuolar Rupture Caused by Intracellular Pathogens

Published on: June 12, 2013

相关概念视频

GPCRs Regulate Adenylyl Cylase Activity

GPCRs Regulate Adenylyl Cylase Activity

Some GPCRs transmit signals through adenylyl cyclase (AC), a transmembrane enzyme. AC helps synthesize second messenger cyclic adenosine monophosphate (cAMP). AC catalyzes cyclization reaction and converts ATP to cAMP by releasing a pyrophosphate. The pyrophosphate is further hydrolyzed to phosphate by the enzyme pyrophosphatase, which drives cAMP synthesis to completion. However, cAMP is rapidly degraded to 5′ AMP by the enzymes phosphodiesterase (PDE), preventing overstimulation of...