当通过鼻内,肌内或两者的组合进行注射时,VSV载体SARS-CoV-2峰值的有效性
Saina Beitari1, Gerard Agbayani2, Melissa Hewitt3
1Infectious Diseases, Human Health Therapeutics Research Centre, National Research Council Canada, Ottawa, ON, Canada.
Scientific reports
|December 4, 2023
概括
用VSV-SARS-CoV-2尖端疫苗进行鼻内或联合鼻内/肌内免疫,可以引起强大的免疫反应和对小鼠和仓鼠的COVID-19变体的保护.
科学领域:
- 疫苗学和病毒学
- 免疫学 免疫学 免疫学
- 传染性疾病 传染性疾病
背景情况:
- 膀性口腔炎病毒 (VSV) 载体疫苗可以伪型化以改变细胞热带性.
- 肌肉内注射是常见的疫苗注射途径,但ACE2在骨肌肉上的表达是未知的.
- 给药途径显著影响疫苗诱导的局部和全身免疫反应.
研究的目的:
- 通过使用不同的主要增强免疫路径,研究VSV-SARS-CoV-2尖端疫苗的免疫性和有效性.
- 为了比较鼻内免疫 (IN),肌内免疫 (IM) 和联合免疫策略.
- 评估针对SARS-CoV-2变种的疫苗有效性.
主要方法:
- 给K18模拟小鼠进行了主要增强免疫方案 (IM,IN,IM+IN,IN+IN).
- 测量了幽默 (IgG,IgA) 和细胞 (T细胞) 免疫反应.
- 仓鼠通过IN或IM+IN路线接收了两剂,并被SARS-CoV-2变种 (阿尔法,三角形) 挑战.
主要成果:
- 在小鼠中,IM+IN和IN+IN主要增强疗法诱导了强烈的尖端特异性IgG,IgA和T细胞反应.
- 通过IN或IM+IN接种疫苗的仓鼠显示出对SARS-CoV-2Alpha和Delta变种的显著保护.
- 在年轻和年长的仓鼠中都观察到保护.
结论:
- 免疫接种途径极大地影响了VSV载体疫苗的疗效.
- 内鼻和联合IM+IN路线对于VSV-SARS-CoV-2尖端疫苗接种非常有效.
- 该平台展示了保护当前和新出现的SARS-CoV-2变种的潜力.
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