相关实验视频

Updated: Jul 9, 2025

Super-Resolution Imaging of Bacterial Secreted Proteins Using Genetic Code Expansion

Super-Resolution Imaging of Bacterial Secreted Proteins Using Genetic Code Expansion

Published on: February 10, 2023

在大肠杆菌cccdB毒素基因中单个同名编码子替代的强现型效应的分子基础

Priyanka Bajaj^1,2, Munmun Bhasin¹, Raghavan Varadarajan³

¹Molecular Biophysics Unit, Indian Institute of Science, Bangalore, 560012, India.

|December 4, 2023

概括

在ccdB基因的同义突变可以显著影响细胞生长和蛋白质功能,与典型的预期相反. 诸如代码子位置和tRNA丰富度等因素影响这些效应,为基因表达优化提供了洞察力.

科学领域:

分子生物学分子生物学
遗传学是一个遗传学.
生物信息学是一种生物信息学.

背景情况:

同义代码子突变通常对基因功能的影响很小.
这项研究调查了ccdB细胞毒素基因在毒素-抗毒素 (TA) 操作语境内的约200个同名突变.
TA操作子的特点是自我调节,这被利用来评估CcdAB复合体形成.

研究的目的:

为了估计ccdB基因中约200个同名编码突变对细胞生长的影响.
了解影响同名突变表型效应的各种因素的相互作用.
为优化大肠杆菌中异质基因表达提供见解.

主要方法:

ccdB基因的系统突变使得代码子退化.
使用转化为大肠杆菌菌株和深度测序的表型分析.
使用RelE毒素记者菌株来量化CcdAB复合体的形成.

主要成果:

在N-终端翻译启动区域的同名突变具有最显著的表型效应.
现型结果是由编码子位置,使用,tRNA丰富度,RNA结构和进化保护的复杂相互作用决定的.
N端突变增强了翻译启动,揭示了对蛋白质折叠和终结的影响.
暂停点的引入影响了转化速率和潜在的蛋白质折叠动力学.

结论:

同义突变可以通过多种机制调节基因功能.
这些发现有助于优化大肠杆菌中异质基因表达的优化.
这项研究阐明了由同名突变引起的基因表达改变背后的分子机制.

关键词:

延伸延伸延伸延伸延伸进化进化进化进化进化进化进化健身适应适应适应适应错误折叠没有错误折叠在Operon上操作.核糖体密码子的使用方式

更多相关视频

Mapping Bacterial Functional Networks and Pathways in Escherichia Coli using Synthetic Genetic Arrays

Mapping Bacterial Functional Networks and Pathways in Escherichia Coli using Synthetic Genetic Arrays

Published on: November 12, 2012

Mutagenesis and Functional Selection Protocols for Directed Evolution of Proteins in E. coli

Mutagenesis and Functional Selection Protocols for Directed Evolution of Proteins in E. coli

Published on: March 16, 2011

相关概念视频

From DNA to Protein

From DNA to Protein

The flow of genetic information in cells from DNA to mRNA to protein is described by the central dogma, which states that genes specify the sequence of mRNAs, which in turn specify the sequence of amino acids making up all proteins. The decoding of one molecule to another is performed by specific proteins and RNAs. Because the information stored in DNA is so central to cellular function, it makes intuitive sense that the cell would make mRNA copies of this information for protein synthesis...

Translation

Translation

Lesson: Translation
Translation is the process of synthesizing proteins from the genetic information carried by messenger RNA (mRNA). Following transcription, it constitutes the final step in the expression of genes. This process is carried out by ribosomes, complexes of protein and specialized RNA molecules. Ribosomes, transfer RNA (tRNA), and other proteins produce a chain of amino acids—the polypeptide—as the end product of translation.
Translation Produces the Building Blocks of...

The Central Dogma

The Central Dogma

Exon Recombination

Exon Recombination

The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes.
Exon shuffling follows “splice frame rules.” Each exon...

Mutations

Mutations

Genome Copying Errors

Genome Copying Errors

DNA replication is a well-evolved process that copies millions of base pairs with high fidelity during each cell division. Occasionally a wrong base or a long stretch of wrong bases may get added to the daughter strands. If the errors are left unchecked, cells might accumulate several mutations that might endanger their survival. Therefore, the copying errors are checked and repaired at three levels.