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通过CRISPR-Cas9系统有针对性地消除万科米辛耐药性基因vanA

Shuan Tao^1,2, Chunwei Hu³, Yewei Fang¹

¹Department of Clinical Laboratory, The First Affiliated Hospital of Ningbo University, No 59. Liuting Road, Haishu District, Ningbo, 315010, China.

BMC microbiology

|December 4, 2023

概括

克里斯普尔-卡斯9系统有效地消除了细菌中的万素耐药性 (vanA) 质粒,降低了抗菌素耐药性,并阻止了基因传播. 这种方法显示出对抗耐药性病原体的潜力.

科学领域:

分子生物学分子生物学
抗微生物耐药性研究研究
基因编辑技术的技术

背景情况:

范A基因在细菌中赋予了万科米辛耐药性,这是一个日益严重的公共卫生问题.
带有vanA的等离子体的水平基因转移促进了抗菌素耐药性的快速传播.

研究的目的:

调查CRISPR-Cas9系统在消除vanA含有等离子体方面的有效性.
评估CRISPR-Cas9的潜力,作为一种减少万科米辛耐药性的策略.

主要方法:

设计的特定单导向RNA (sgRNAs) 针对vanA基因.
为vanA基因分裂构建了一个CRISPR-Cas9等离子体系统.
使用化学转化和结合用于输送.
通过盘子计数,PCR和qPCR评估了等离子体的消除.
使用易感性测试评估细菌耐药性表型的变化.

主要成果:

构建的CRISPR-Cas9等离子体系统有效地清除了含有vanA的等离子体.
细菌对万科米的耐药性显著降低了等离子体清除后.
该CRISPR-Cas9系统有效地阻止了含有vanA的等离子体的水平转移.

结论:

CRISPR-Cas9系统成功实现了等离子体清除和降低了抗菌素耐药性.
这种基因编辑策略具有很大的潜力,可以对抗vanA基因的传播.
提供了利用CRISPR-Cas9作为辅助抗生素治疗的基础.

关键词:

抗微生物耐药性抗微生物耐药性克里斯普尔-Cas9是什么意思肠球菌 (Enterococcus) 是一个细菌.瓦纳 (vanA) 的意思是

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