巨细胞动脉炎中的衰老细胞表现出一种炎症表型,通过依赖IL-6的途径参与组织损伤
Dimitris Veroutis1, Ourania D Argyropoulou2, Andreas V Goules2,3,4
1Molecular Carcinogenesis Group, Department of Histology and Embryology, Medical School, National and Kapodistrian University of Athens, Athens, Greece.
Annals of the rheumatic diseases
|December 5, 2023
概括
细胞衰老,衰老的标志,在巨细胞动脉炎 (GCA) 中发挥作用. GCA动脉中的衰老细胞与炎症有关,这表明它们参与了疾病过程.
科学领域:
- 类风湿病学 类风湿病学
- 免疫学 免疫学 免疫学
- 细胞生物学 细胞生物学
背景情况:
- 巨细胞动脉炎 (GCA) 是一种主要影响老年人的炎症性血管病.
- 年龄是GCA的主要风险因素,这表明细胞衰老在其发病过程中可能发挥作用.
- 细胞衰老,一种不可逆转的细胞循环停止状态,与衰老和炎症有关.
研究的目的:
- 调查从GCA患者的动脉活检 (TAB) 中老化的细胞的存在和起源.
- 确定衰老细胞与炎症标志物和GCA中的疾病严重程度的关联.
- 探索IL-6在诱导GCA细胞衰老中的潜在作用.
主要方法:
- 从GCA患者,多发性肌痛性风湿症 (PMR) 患者和非GCA/非PMR对照对象的关动脉活检 (TAB) 的分析.
- 使用多标记算法识别衰老细胞并评估它们的组织起源 (纤维细胞,巨细胞,内皮细胞).
- 在衰老细胞中量化IL-6和MMP-9的表达,并研究它们与血管炎症的关联. 在体外实验中评估了IL-6诱导的衰老.
主要成果:
- 与PMR动脉 (2.66%) 相比,GCA动脉中衰老细胞的数量显著增加 (9.50%).
- 衰老细胞主要来自GCA中的纤维细胞,巨细胞和内皮细胞.
- 阳性IL-6衰老细胞与GCA中的血管炎症程度相关,以及GCA超标在体外诱导衰老.
结论:
- 在GCA动脉中存在表现出炎症表型的衰老细胞.
- 这些衰老细胞与GCA中的炎症透物有关,使它们与疾病病原发生有关.
- 准IL-6通路可能是GCA的潜在治疗策略.
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