光遗传学揭示了支持细胞在向和从小鼠耳中的外发细胞的力传输中的作用
Victoria A Lukashkina1, Snezana Levic1,2, Patricio Simões1
1Sensory Neuroscience Research Group, School of Applied Sciences, University of Brighton, Brighton BN2 4GJ, United Kingdom.
概括
用光激活耳中的支细胞抑制了放大,并减少了内毛细胞的激发,揭示了这些细胞是治疗听力损失的潜在目标.
科学领域:
- 听觉神经科学 听觉神经科学
- 细胞电生理学 细胞电生理学
- 机械生物学 机械生物学
背景情况:
- 外皮毛细胞 (OHCs) 是尾中的关键机电传感器,与像Deiters细胞 (DCs) 和外柱细胞 (OPCs) 这样的支持细胞相互作用.
- 这种由支细胞支架介导的相互作用对于听觉灵敏度,频率选择性和信号传输到听觉神经至关重要.
研究的目的:
- 通过使用光遗传学选择性激活它们来研究DCs和OPCs在耳功能中的作用.
- 了解操纵这些支持细胞如何影响耳放大和听觉神经反应.
主要方法:
- 在小鼠的DC和OPC中,一种光门的化物,哈洛罗多普辛 (HOP) 的条件表达.
- 测量细胞外受体潜力 (ERP) 和听觉神经化合物作用潜力 (CAP).
- 对HOP表达小鼠与HOP淘汰 littermates的反应进行比较.
主要成果:
- 光激活的DC和OPC的超极化通过改变反时间来抑制耳放大.
- 基底膜对声音的反应在所有测试的水平和频率上都发生了变化.
- 内毛细胞 (IHC) 激发减少,影响听觉神经活动.
结论:
- 迪特尔细胞和外侧支柱细胞在耳放大和声音处理中发挥着关键作用.
- 对支细胞的选择性操纵会影响耳机械和IHC功能.
- DC和OPC代表了噪音引起的听力损失等疾病的潜在治疗点.
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