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阿尔特纳里亚TeA毒素激活了叶绿体逆行信号通路,以促进JA-依赖的致病性

Jiale Shi¹, He Wang¹, Mengping Li²

¹Weed Research Laboratory, Nanjing Agricultural University, Nanjing 210095, China.

Plant communications

|December 5, 2023

概括

来自Alternaria真菌的亚酸 (TeA) 触发了叶绿体单片氧 (1O2) 生产,激活了EXECUTER1 (EX1) 信号. 这一途径驱动植物细胞死亡和Arabidopsis的疾病发展.

科学领域:

植物病理学植物病理学
分子植物微生物相互作用.
光合作用研究研究光合作用.

背景情况:

叶绿体是植物病原体相互作用的关键地点.
十亚酸 (TeA) 是来自Alternaria的一种真菌毒素,它破坏光合作用并产生单片氧 (1O2).
1O2参与了叶绿体到核的逆行信号和植物损伤.

研究的目的:

为了阐明TeA诱导的Arabidopsis thaliana病原性的分子机制.
研究EXECUTER1 (EX1) 在TeA诱导的信号和植物防御反应中的作用.
了解在真菌感染期间的1O2信号传递和莉酸 (JA) 途径之间的联系.

主要方法:

对具有改变EX1功能 (EX1-W643A,ex1删除) 的阿拉比多普西斯突变物进行遗传分析.
测量 1O2 生产和 1O2 响应基因表达.
对素酸 (JA) 合成和信号基因表达的分析.

主要成果:

Alternaria alternata利用EX1/EX2介导的逆行信号,由TeA诱导的1O2触发,以引起死细胞损伤.
对1O2敏感的EX1-W643残留物或EX1的传感器域的突变会损害对1O2敏感的核基因表达和病变的发展.
TeA迅速诱导JA合成和信号基因,EX1介导的信号对于1O2到JA信号级联至关重要.

结论:

对于Alternaria alternata的病原性来说,EX1依赖的1O2信号是必不可少的.
这项研究揭示了一个信号级联,TeA诱导的1O2激活EX1,这反过来又促进了JA-依赖的细胞死亡.
这项工作加深了我们对植物病原菌如何利用叶绿体信号传递来发展疾病的理解.

关键词:

另类病原体 Alternaria 的病原体.这是一种TeA菌毒素.类塑料是一种类塑料.斯酸是一种的酸.逆行信号传输方式一个单一的氧气.

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