乙基异硫酸作为一种新型抗真菌剂,用于对抗白虫
Shivani Balasaheb Patil1, Rakesh Kumar Sharma2, Tanjila Chandsaheb Gavandi1
1Department of Stem Cell and Regenerative Medicine, Medical Biotechnology, Centre for Interdisciplinary Research, D. Y. Patil Education Society (Deemed to be University), Kolhapur, Maharashtra, 416003, India.
Current microbiology
|December 5, 2023
概括
乙异硫酸 (EITC) 通过抑制生长,毒性和生物膜形成,有效地对抗 Candida albicans. 这项研究揭示了EITC.
科学领域:
- 菌类学 菌类学是指菌类学.
- 生物化学 生物化学
- 药理学 药理学 是一个学科.
背景情况:
- кандидоз的发病率和死亡率正在上升,特别是在免疫受损的个体中.
- 葡萄糖酸盐 (GLS) 衍生物显示出抗真菌的潜力,但乙异硫酸盐 (EITC) 对抗白菌的机制尚不清楚.
- 需要新的抗真菌药物来对抗耐药的Candida菌株.
研究的目的:
- 阐明乙基异硫酸 (EITC) 的抗甘地达菌疗效和作用机制.
- 研究EITC对Candida albicans浮游生物生长,毒性因子和生物膜形成的影响.
- 评估EITC的体外和体内活性以及潜在的毒性.
主要方法:
- 在体外测试测量浮游生物生长抑制,毒性因子抑制 (形态发生,粘附,生物膜形成),厄戈斯特醇生物合成,细胞循环停止和氧化应激.
- 对参与形态发生的关键信号通路基因的基因表达分析.
- 使用丝虫模型进行体内毒性和疗效评估.
主要成果:
- 在较低度 (0.0312 mg/ml) 时,EITC有效抑制了Candida albicans浮游生物的生长 (0.5 mg/ml) 和关键毒性因子.
- EITC阻断了厄戈斯特的生物合成,诱导了S相停止,引起了ROS依赖的细胞死亡和DNA碎片化.
- EITC调节基因表达,对TUP1,MIG1和NRG1进行上调,同时对PDE2和CEK1进行下调,从而抑制酵母转基因. 在体内研究表明,EITC在0.5mg/ml的剂量下对C. albicans的疗效,并观察到有毒性.
结论:
- 乙异硫酸 (EITC) 通过多种机制对Candida albicans表现出显著的抗真菌活性.
- EITC有效地抑制了真菌生长,毒性和生物膜形成,提供了潜在的替代治疗方法.
- 建议进行组合研究,以减少EITC的最小抑制度 (MIC) 和毒性,以加强临床应用.
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