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Updated: Jul 9, 2025

Indel Detection following CRISPR/Cas9 Mutagenesis using High-resolution Melt Analysis in the Mosquito Aedes aegypti
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一种PCR诱导的突变-限制片段长度多态化方法,用于检测CRISPR诱导的体

Lydia Angelopoulou1, Electra Stylianopoulou1, Konstantinos Tegopoulos1

  • 1Department of Molecular Biology and Genetics, Democritus University of Thrace, Alexandroupolis, Greece.

The CRISPR journal
|December 5, 2023
PubMed
概括

我们开发了一种新的方法,PCR诱导的突变发生-限制片段长度多态化 (PIM-RFLP),以有效地识别基因编辑的克隆. 这种具有成本效益的技术超过了桑格测序,用于在CRISPR应用中检测完全突变的克隆.

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Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
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