保护性微生物,病原体和宿主之间的复杂的三角相互作用决定了超生物体的适应性
Hanne Griem-Krey1, Carola Petersen1, Inga K Hamerich1
1Department of Evolutionary Ecology and Genetics, Kiel University, Kiel 24118, Germany.
Proceedings. Biological sciences
|December 5, 2023
概括
主体免疫和保护性微生物相互作用,塑造超生物体的健康状况. 宿主免疫反应在病原体压力下比保护性微生物更为关键,但保护性微生物仍然可以增强生存能力. 免疫力受损会逆转保护作用.
科学领域:
- 微生物学 微生物学
- 宿主-微生物相互作用
- 超生物生物生物学 超生物生物学
背景情况:
- 宿主微生物群显著影响宿主生物学,包括对病原体的保护.
- 主体 - 病原体和主体 - 微生物群的相互作用通常被单独研究.
- 主体免疫,保护性微生物和病原体对超生物体健康的同时影响仍然不清楚.
研究的目的:
- 为了研究宿主 (Caenorhabditis elegans),保护性共生体 (Pseudomonas lurida MYb11) 和病原体 (Bacillus thuringiensis Bt679) 之间的三角相互作用.
- 确定宿主免疫和保护性共生体殖民如何影响在病原体压力下超生物体的健康状况.
- 阐明特定宿主免疫通路的作用,如p38 MAPK信号传递,在调解这些相互作用.
主要方法:
- 使用了一个模型系统,包括线虫Caenorhabditis elegans,细菌Pseudomonas lurida MYb11和细菌Bacillus thuringiensis Bt679.9.
- 雇佣的C. elegans突变体具有免疫力和/或微生物殖民能力的改变.
- 评估元生物体的适应性,生存率以及特定信号通路 (p38 MAPK) 对宿主微生物动态的影响.
主要成果:
- 发现宿主免疫能力对超生物体的健康状况比在病原体压力下保护性微生物殖民更有影响.
- 在大多数实验条件下,保护性共生体MYb11始终改善了宿主健康状况.
- 干扰p38 MAPK信号取消了MYb11的保护作用,导致线虫在Bt679感染时的存活率降低.
结论:
- 宿主免疫在管理甲基生物体内的保护性微生物和病原体之间的复杂相互作用方面发挥着至关重要的作用.
- 保护性共生体可以提高宿主的健康状况,但它们的有效性取决于宿主的免疫状态.
- 了解宿主免疫路径对于预测和操纵超生物体对微生物挑战的反应至关重要.
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