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相关概念视频

Gas Chromatography: Introduction01:13

Gas Chromatography: Introduction

2.0K
Gas chromatography (GC) is a technique for separating and analyzing volatile compounds in a sample. Its primary purpose is to identify and quantify components in complex mixtures, making it essential in fields such as environmental analysis, pharmaceuticals, and petrochemicals. GC is also called vapor-phase chromatography (VPC) or gas-liquid partition chromatography (GLPC).
In GC,  a sample is vaporized and mixed with an inert carrier gas (the mobile phase), which transports it through a...
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Gas Chromatography–Mass Spectrometry (GC–MS)01:14

Gas Chromatography–Mass Spectrometry (GC–MS)

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Gas chromatography–mass spectrometry (GC–MS) is the combination of analytical techniques of gas chromatography and mass spectrometry in a single instrument for analyzing a mixture of compounds. The gas chromatograph separates the compounds in the mixture, and the mass spectrometer analyzes each compound separately to determine the molecular masses and molecular structures.
A gas chromatograph consists of a long, narrow capillary column with a polysiloxane coating on the inner wall....
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Case Studies01:22

Case Studies

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There are many research methods available to psychologists in their efforts to understand, describe, and explain behavior and the cognitive and biological processes that underlie it.
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Gas Chromatography: Types of Columns and Stationary Phases01:17

Gas Chromatography: Types of Columns and Stationary Phases

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Gas chromatography (GC) relies on stationary phases to separate and analyze components in a sample. There are two main types of stationary phases: liquid and solid. Liquid stationary phases are non-volatile, thermally stable, and chemically inert liquids coated onto the column. Solid stationary phases are particles of adsorbent material, such as silica gel or molecular sieves.
For an analyte to remain on the column for a sufficient amount of time, it must exhibit some level of compatibility (or...
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Principles Of Column Chromatography01:13

Principles Of Column Chromatography

6.8K
The chromatography technique was first invented in 1901 by Michael S. Tswett, a Russian botanist, to separate plant pigments using organic solvents. Further, in 1941, Archer John Porter Martin and R. L. M. Synge modified the technique by packing silica gel into a column. A mixture of amino acids was then separated on the packed column using chloroform and water mixture as the mobile phase. This was the first report on column chromatography. At present, column chromatography is a widely used...
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Chromatography: Introduction01:10

Chromatography: Introduction

4.4K
Chromatography is a technique used to separate compounds based on differences of partitioning between two phases, the stationary phase and the mobile phase.
The phase in which the compounds linger or on which the compounds adsorb is called the stationary phase, whereas the mobile phase is the solvent that carries the solutes to be analyzed. In traditional column chromatography, the mixture flows through the stationary phase, and the compounds partition between the stationary and mobile phases...
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相关实验视频

Updated: Jul 9, 2025

Atom Probe Tomography Studies on the CuIn,GaSe2 Grain Boundaries
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Atom Probe Tomography Studies on the CuIn,GaSe2 Grain Boundaries

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照明未经研究的GPCR-ome.

Sreeparna Majumdar1, Yi-Ting Chiu1, Julie E Pickett1

  • 1Department of Pharmacology, UNC Chapel Hill School of Medicine, Chapel Hill, NC 27599, USA.

Drug discovery today
|December 5, 2023
PubMed
概括

"照亮可使用药物的基因组"项目提供了研究不足的G蛋白结合受体 (GPCRs) 的工具. 这些资源正在揭示它们的功能和药物潜力.

科学领域:

  • 药理学和分子生物学
  • 基因组学和生物信息学

背景情况:

  • G蛋白结合受体 (GPCRs) 是主要的药物标类别,超过30%的批准药物对它们起作用.
  • 然而,在超过800个人类GPCR中,很大一部分仍未被充分理解,通常被称为"黑暗"受体.
  • 启发可使用药物的基因组 (IDG) 计划旨在解决这一知识差距.

研究的目的:

  • 利用IDG项目产生的资源,探索未经充分研究的GPCRs的功能和药用性.
  • 提供关于"暗"GPCR的IDG项目中获得的工具和见解的全面概述.

主要方法:

  • 使用IDG项目开发的一套工具,包括高通量选试验 (例如PRESTO-TANGO,TRUPATH).
  • 利用大型化学图书馆,如ZINC虚拟图书馆,包含数十亿个小分子.
  • 采用结构生物学技术来解决孤儿GPCR结构,并利用基因模型,如GPCR敲进小鼠.

主要成果:

  • IDG项目成功地产生了各种工具和资源,促进了对"黑暗"GPCR的研究.
  • 这些工具为以前未被描述的GPCRs的功能作用和治疗潜力提供了新的见解.
  • 联合努力已经开始揭示这些研究不足的受体的生物学和药物可用性景观.
关键词:
敲进的小鼠 敲进的小鼠基于细胞的测定.化-EM结构的结构.发现药物的发现.高吞吐量选的高吞吐量选照亮可使用药物的基因组 (IDG).分子/化学探针的探测器.纳米体是一个纳米体.孤儿 GPCRs 的使用情况

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结论:

  • IDG项目的资源对于推动我们对"暗"GPCRs的理解至关重要.
  • 这些工具有助于识别新的治疗点和开发用于广泛疾病的新药.
  • 使用这些先进工具对GPCR的持续探索有望扩大可用药物向的目录.