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工程氨酸氧甲基转移酶,以在大肠杆菌中有效合成L-氨酸

Zixin Teng1, Xuewei Pan1, Yunran Liu1

  • 1Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, Jiangsu, China; Yixing Institute of Food and Biotechnology Co., Ltd, Yixing 214200, China.

Bioresource technology
|December 5, 2023
PubMed
概括

改造的氨酸氧甲基转移酶 (SHMT) 显著提高了L-氨酸的产生. 这个蜂工厂实现了创纪录的106.06g/L L-氨酸产量,克服了工业限制.

关键词:
同进化的分析.这是一种L-氨酸.蛋白质工程是一种蛋白质工程.血清氧甲基转移酶的氧甲基转移酶.在mRNA的稳定性上.

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Strain improvement is a foundational strategy in industrial microbiology aimed at maximizing microbial productivity, particularly because natural isolates typically yield commercially valuable products in very low concentrations. Although optimizing the culture medium and environmental conditions can improve yields, these adjustments are inherently limited by the organism’s genetic potential. As a result, the focus shifts toward genetic modifications to enhance biosynthetic capacity. The...