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新生儿组织的表观遗传变异在婴儿怀孕使用容量化-体外成熟与体外受精在体外受精

Laura Saucedo-Cuevas¹, Mai P Q Ma², Anh H Le²

¹Follicle Biology Laboratory, UZ Brussel, Vrije Universiteit Brussel, Brussel, Belgium.

Fertility and sterility

|December 5, 2023

概括

与传统体外受精 (IVF) 相比,容量体外成熟 (CAPA-IVM) 在新生儿中显示出最小的表观遗传变异. 这项研究调查了胎盘,带血液和口腔涂抹中的DNA甲基化概况.

科学领域:

生殖医学生殖医学
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.
基因组学就是基因组学.

背景情况:

像体外受精 (IVF) 这样的辅助生殖技术 (ART) 被广泛使用.
不同的ART协议的长期表观遗传效应,包括体外成熟 (IVM),需要进一步研究.
容量化IVM (CAPA-IVM) 是一种修改后的IVM技术.

研究的目的:

为了比较通过CAPA-IVM怀孕的新生儿的全球DNA甲基化概况与传统的试管婴儿.
评估胎盘,带血和新生儿口腔涂抹中的表观遗传变化.

主要方法:

一个随机对照试验比较CAPA-IVM和IVF在患有PCOS或高毛囊数量的女性中.
在58名新生儿的样本上使用Illumina Infinium MethylationEPIC BeadChip进行全基因组DNA甲基化分析.
两组之间的甲基化变异性比较.

主要成果:

在CAPA-IVM和IVF组之间的新生儿口腔涂抹中没有观察到DNA甲基化的显著差异.
在带血液中的一个CpG部位显示了CAPA-IVM组的甲基化增加.
胎盘中的五个CpG位点显示甲基化发生变化,CAPA-IVM组的胎盘和带血液样本中发现甲基化变异性增加.

结论:

与传统试管婴儿相比,CAPA-IVM与带血,胎盘组织和新生儿口腔涂抹的最小表观遗传变异有关.
这些发现表明CAPA-IVM的潜在有利的表观遗传特征.

关键词:

容量 - 在体外成熟.通过DNA甲基化.印记的基因印记的基因新生儿组织新生儿组织.卵子细胞卵子细胞

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