相关实验视频

Updated: Jul 9, 2025

Chitosan/Interfering RNA Nanoparticle Mediated Gene Silencing in Disease Vector Mosquito Larvae

Chitosan/Interfering RNA Nanoparticle Mediated Gene Silencing in Disease Vector Mosquito Larvae

Published on: March 25, 2015

通过调节Keap1/Nrf2/HO-1通路,SeMet可以缓解LPS诱导的卵腺死介导炎症

Dan Chen¹, Wenying Sun¹, Huanyi Liu¹

¹College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, PR China.

Archives of biochemistry and biophysics

|December 5, 2023

概括

有机 (氨酸) 通过调节Keap1/Nrf2/HO-1通路,在蛋腺组织中减轻氧化应激和亡,保护产母免受脂多糖诱导的炎症.

科学领域:

动物科学动物科学
营养生物化学营养生物化学
兽医病理学兽医病理学

背景情况:

脂聚糖 (LPS) 暴露会导致各种组织的炎症.
(Se) 对于减轻炎症损伤至关重要,有机形式如甲 (SeMet) 提供更好的吸收和更低的毒性.
蛋腺容易受到LPS引起的损伤,影响着产母的生产力.

研究的目的:

调查SeMet对LPS引起的对Hy-Line Brown产母蛋腺组织的保护作用.
阐明潜在的分子机制,包括氧化应激,亡和炎症途径.

主要方法:

棕色的Hy-Line产卵被分配到对照组,LPS,SeMet和SeMet + LPS组.
组织病理学检查 (H&E染色,TEM) 评估了组织损伤.
测量了氧化应激标志物 (MDA,SOD,CAT,GPX,GSH) 的含量.
使用qRT-PCR和西式涂抹,评估了Keap1/Nrf2/HO-1通路,亡 (RIPK1,RIPK3,MLKL) 和炎症 (TNF-α,COX-2,NF-κB) 的mRNA和蛋白质水平.

主要成果:

随着LPS的挑战,恶性甲酸 (MDA) 和炎症标志物 (Keap1,RIPK1,RIPK3,MLKL,TNF-α,COX-2,NF-κB) 增加,同时降低了抗氧化酶活动 (SOD,CAT,GPX) 和谷氨 (GSH).
此外,LPS还降低了Nrf2通路组件 (Nrf2,HO-1,NQO1) 和亲细胞亡标志物Caspase-8的水平.
补充SeMet有效地逆转了这些LPS诱导的变化,恢复了抗氧化剂状态,并通过Keap1/Nrf2/HO-1通路抑制了亡和炎症.

结论:

SeMet可以缓解产卵母LPS诱导的卵腺组织损伤.
保护机制包括调节Keap1/Nrf2/HO-1通路,从而减少死细胞灭绝中介的炎症和氧化应激.
通过减轻LPS诱导的炎症损伤,SeMet显示了改善产母健康和产量的潜力.

关键词:

炎症反应是一种炎症反应.尸体灭 (Necroptosis) 是一种死亡的过程.氧化应激是一种氧化应激.胺氨酸是一种胺氨酸.

更多相关视频

Monitoring On-Target Signaling Responses in Larval Zebrafish - Z-REX Unmasks Precise Mechanisms of Electrophilic Drugs and Metabolites

Monitoring On-Target Signaling Responses in Larval Zebrafish - Z-REX Unmasks Precise Mechanisms of Electrophilic Drugs and Metabolites

Published on: June 2, 2023

Bronchoalveolar Lavage Exosomes in Lipopolysaccharide-induced Septic Lung Injury

Bronchoalveolar Lavage Exosomes in Lipopolysaccharide-induced Septic Lung Injury

Published on: May 21, 2018

相关概念视频

Necrosis

Necrosis

Necrosis is considered as an “accidental” or unexpected form of cell death that ends in cell lysis. The first noticeable mention of “necrosis” was in 1859 when Rudolf Virchow used this term to describe advanced tissue breakdown in his compilation titled “Cell Pathology”.
Morphological Manifestations of Necrosis
Necrotic cells show different types of morphological appearance depending on the type of tissue and infection. In coagulative necrosis, cells become...

Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal

Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal

Erythropoietin-producing hepatocellular carcinoma receptor (Eph) and its ligand, Eph receptor-interacting protein (Ephrin) were first discovered in the human carcinoma cell line, hence the name. Ephrin-Eph interaction guides cells to reach their appropriate location in adult tissues. They also play an essential role in the immune system by helping in immune cell migration, adhesion, and activation. Based on their structure and function, Eph is divided into two classes — EphA and EphB.

Hedgehog Signaling Pathway

Hedgehog Signaling Pathway

The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...