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优化对低强度脉冲超声波的辐射条件,以提高内皮氧化合成酶的调节

Daiki Ouchi¹, Shohei Mori¹, Mototaka Arakawa^2,3

¹Graduate School of Engineering, Tohoku University, 6-6-05 Aoba, Aramaki, Aoba-Ku, Sendai, Miyagi, 980-8579, Japan.

Journal of medical ultrasonics (2001)

|December 5, 2023

概括

低强度脉冲超声波 (LIPUS) 治疗显示出通过增强血管生长来治疗缺血性心脏病的潜力. 这项研究确定了低于最佳的超声波条件,以促进内皮氧化合成酶 (eNOS) 表达.

科学领域:

心血管研究研究心血管研究
生物医学工程生物医学工程
再生医学是一种再生医学.

背景情况:

缺血性心脏病仍然是全球死亡的主要原因.
目前用于心肌缺血的治疗方法存在局限性.
低强度脉冲超声波 (LIPUS) 治疗是一种有前途的微创疗法.

研究的目的:

开发一种使用LIPUS.LIPUS治疗缺血性心脏病的微创治疗方法.
通过血管生成证明LIPUS疗法在改善心肌缺血的疗效.
为了确定促进内皮氧化合成酶 (eNOS) 表达的最佳LIPUS参数.

主要方法:

研究了五个关键的超声波辐射参数.
利用人类冠状动脉内皮细胞 (HCAEC) 来评估eNOS表达.
定义了一个新的指数,功率×总循环,以将LIPUS参数与eNOS表达式相关联.

主要成果:

确定了低于最佳的LIPUS条件,包括1MHz频率,500kPa声压,20分钟持续时间,32-48微秒脉冲持续时间和320微秒脉冲重复时间.
使用拟议的索引,证明了 LIPUS 参数与 eNOS 表达式之间的统一关系.
建立了优化LIPUS治疗心肌血管生成的基础.

结论:

确定了低于最佳的超声波照射条件,以促进HCAEC中的eNOS表达.
提供了LIPUS参数和血管生成分子表达之间的关系的见解.
奠定了进一步研究心血管应用LIPUS优化的基础.

关键词:

血管新生的产生.内氧化合成酶是内皮氧化合成酶.人的冠状动脉内皮细胞是冠状动脉内皮细胞.低强度脉冲超声波疗法是一种低强度脉冲超声波疗法.

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