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在发育中的胎儿丸中,FGF独立的MEK1/2信号对于小鼠的雄性生殖系差异化至关重要

Rheannon O Blücher¹, Rachel S Lim², Ellen G Jarred¹

¹Centre for Reproductive Health, Hudson Institute of Medical Research and Department of Molecular and Translational Science, Monash University, Clayton, VIC, 3168, Australia.

|December 5, 2023

概括

在丸发育过程中,MEK1/2信号对于男性生殖系的分化至关重要,而FGF信号则起到更有限的作用. 这一发现促进了对男性生育能力和丸发育的理解.

科学领域:

生殖生物学生殖生物学
发展生物学发展生物学
分子内分泌学分子内分泌学

背景情况:

干扰生殖线分化和丸发育与不孕症和丸癌症有关.
SRY和SOX9诱导FGF9,促进塞尔托利细胞分化和丸发育.
通过MAPK信号传递,FGF9影响男性生殖线分化的机制尚不清楚.

研究的目的:

研究FGF9介导的MAPK信号在男性生殖线发育中的作用.
要确定MEK1/2抑制是否影响生殖细胞分化和丸发育.

主要方法:

在胎儿小鼠丸中抑制FGF或MEK1/2信号.
对塞尔托利细胞增殖,组织和生殖细胞定位的分析.
评估生殖细胞线性阻断和男性生殖系关键标志物 (DPPA4,DNMT3L) 的表达.

主要成果:

抑制MEK1/2干扰了生殖细胞的线粒捕获,并阻止了DPPA4和DNMT3L的上调.
抑制FGF降低了塞尔托利细胞的增殖,但没有影响生殖系标记物的表达或线粒性停止.
抑制FGF和MEK1/2都改变了胚胎细胞中的干细胞和癌症相关基因.

结论:

在丸发育过程中,MEK1/2信号对于男性生殖系的分化至关重要.
与MEK1/2.2.相比,FGF信号在男性生殖线差异化中的作用较为有限.
其他配体可能利用MEK1/2促进雄性生殖线分化,需要进一步研究.

关键词:

FGF9 联邦政府财政基金胎儿的生殖腺是什么细菌细胞是细菌细胞的组成部分.生殖线发展的生殖线发展.细菌线线线粒细胞的停止.这就是MAP激酶.一个MEK1/2的人.性差异化性别差异化测试 (Testis) 是一个测试.

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