在慢性乙型肝炎的非酒精性脂肪肝炎中HIGD1A的功能作用
Min-Ran Li1, Jin-Zhong Li1, De-Hua Wang2
1Division of Infectious Disease, The First Affiliated Hospital of Jinan University, Guangzhou, China.
Scandinavian journal of gastroenterology
|December 6, 2023
概括
该研究发现,HIGD1A在非酒精性脂肪肝炎 (NASH) 患有慢性乙型肝炎 (CHB) 的患者中升高. HIGD1A保护肝细胞免受氧化应激,这表明它可能调节CHB患者的NASH发展.
科学领域:
- 肝病学 肝病学是一种肝病学.
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 非酒精性脂肪性肝病 (NAFLD) 的患病率正在上升,与慢性乙型肝炎 (CHB) 的同时发生率正在增加.
- 在CHB患者中导致非酒精性脂肪肝炎 (NASH) 发展的因素尚不清楚.
研究的目的:
- 调查长非编码RNAs (lncRNAs) 和信使RNAs (mRNAs) 在NASH发展中的作用.
- 分析HIGD1A在肝组织和CHB和NAFLD细胞模型中的功能.
主要方法:
- 来自CHB和NAFLD患者的肝脏活检的全转录组分析 (NASH与非NASH组).
- 对HIGD1A的功能分析,包括HepG2.2.15细胞的淘汰和过度表达.
- 在体内研究使用乙型肝炎病毒 (HBV) 转基因小鼠.
主要成果:
- 与CHB和NAFLD的非NASH患者相比,NASH患者的HIGD1A表达显著更高.
- 细胞中HIGD1A的敲击加剧了细胞亡和线粒体功能障碍;过度表达改善了自由脂肪酸诱导的损伤.
- HIGD1A通过增加谷氨 (GSH) 来减少活性氧物种 (ROS),独立于AMPK/ACC通路.
结论:
- 在CHB模型中,随着NASH相关的炎症而增加HIGD1A表达.
- HIGD1A在肝细胞中表现出对氧化应激的保护作用.
- 在CHB环境中,HIGD1A被认为是NASH病变发生的潜在积极调节者.
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