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代谢水是否控制微生物细胞中的酸氧同位素?

Tal Weiner¹, Federica Tamburini², Nir Keren³

¹The Institute of Earth Sciences, The Hebrew University of Jerusalem, Jerusalem, Israel.

Frontiers in microbiology

|December 6, 2023

概括

代谢水不能控制微生物细胞的水氧同位素比率 (δ¹⁸O). 实验表明O2和细胞酸盐同位素之间没有联系,挑战古气候和碳使用效率理论.

科学领域:

微生物学微生物学
地质化学地质化学
生物地质化学生物地质化学

背景情况:

微生物细胞水的氧同位素比 (δ¹⁸O) 影响细胞酸盐和其他含氧分子.
细胞呼吸过程中产生的代谢水最近被提出用于控制细胞水的同位素比率.

研究的目的:

直接测试代谢水驱动微生物细胞水的 δ¹⁸O 的假设.
评估对古气候重建和微生物碳使用效率测量的影响.

主要方法:

实验对头部空间O2和水的介质水的Δ¹⁸O进行了变化.
测量了细胞酸盐的18O,假设与细胞水的同位素平衡.
利用标记为O水来追踪细胞酸盐中的氧气来源.

主要成果:

没有发现O2和细胞酸盐的δ¹⁸O之间的相关性,反驳了代谢水假设.
细胞酸盐中只有43%的氧气在生长过程中与水平衡.
在细胞内和细胞外水解过程中观察到的同位素效应可能解释不完全平衡.

结论:

代谢水不是微生物细胞水 δ¹⁸O的重要驱动因素.
细胞酸盐氧同位素受到超出简单与水介质平衡的因素的影响.
需要进一步的研究来了解水解对微生物细胞中酸氧同位素的影响.

关键词:

环境水环境水环境水碳使用效率的碳使用效率.代谢用水的代谢用水微生物细胞是微生物细胞.氧气的稳定同位素氧气的稳定同位素

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