相关实验视频

Updated: Jul 9, 2025

Forward Genetics Screens Using Macrophages to Identify Toxoplasma gondii Genes Important for Resistance to IFN-γ-Dependent Cell Autonomous Immunity

Forward Genetics Screens Using Macrophages to Identify Toxoplasma gondii Genes Important for Resistance to IFN-γ-Dependent Cell Autonomous Immunity

Published on: March 12, 2015

毒素诱导T细胞以BATF-依赖的方式产生IL-10

D Alejandro Canaria¹, J Alejandra Rodriguez¹, Luopin Wang²

¹Department of Biological Sciences, Purdue University, West Lafayette, IN, United States.

Frontiers in immunology

|December 6, 2023

概括

毒素转录因子调节T辅助细胞的分化和功能. 它促进了Th2和Treg细胞的发育,并增强了IL-10的产生,帮助炎症解决.

科学领域:

免疫学免疫学免疫学
分子生物学分子生物学
转录因子转录因子

背景情况:

毒素 (高流动性组-盒子转录因子) 对于胸腺T细胞发育至关重要.
毒素还表达在激活的CD4和CD8T细胞,癌症和自身免疫性疾病中.
虽然已知Tox在T卵泡辅助细胞 (TFH) 中的作用,但其在其他T辅助细胞 (Th) 亚型中的功能不明.

研究的目的:

为了研究Tox在TFH细胞以外的各种T辅助 (Th) 细胞亚型中的作用.
探索Tox如何影响激活Th细胞的分化和基因表达特征.
阐明Tox调节Th细胞功能和炎症的分子机制.

主要方法:

在生理上激活的Th亚型中对Tox表达的分析.
在体外研究Tox对Th2和T调控 (Treg) 细胞分化的影响.
对Tox过度表达Th细胞的基因表达分析.
染色体免疫沉 (ChIP) 测试以确定毒素结合点和相互作用的转录因子 (BATF,IRF4,JunB).

主要成果:

毒素异位表达增强了Th2和Treg细胞的体外分化.
毒素过度表达诱导了与Th亚型细胞激活 (Pdcd1),贩运 (Ccl3,Ccl4,Xcl1) 和炎症抑制 (Il10) 相关的基因.
毒素将这些基因的调节区域与BATF,IRF4和JunB结合起来.
毒素诱导的IL-10产生依赖于BATF,而PD-1诱导则不是.

结论:

毒素在调节多种Th细胞亚型的分化和功能方面发挥着重要作用.
毒素通过受调节的基因影响Th细胞化学反应和树突细胞相互作用.
毒素促进IL-10的产生,有助于炎症的解决或预防,特别是在依赖于BATF的方式.

关键词:

在BATF/JUN/IRF4复合体中,在 IL-10 中,IL-10 是 IL-10 的代表.T细胞分化的特异化托克斯 (Tox) 是一种毒素.转录因子 (TF) S 是一个转录因子.

更多相关视频

Mouse Naïve CD4+ T Cell Isolation and In vitro Differentiation into T Cell Subsets

Mouse Naïve CD4+ T Cell Isolation and In vitro Differentiation into T Cell Subsets

Published on: April 16, 2015

Tailoring In Vivo Cytotoxicity Assays to Study Immunodominance in Tumor-specific CD8+ T Cell Responses

Tailoring In Vivo Cytotoxicity Assays to Study Immunodominance in Tumor-specific CD8+ T Cell Responses

Published on: May 6, 2019

相关概念视频

T Cell Types and Functions

T Cell Types and Functions

When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...

Cytotoxic T Cells-mediated Immune Response

Cytotoxic T Cells-mediated Immune Response

Cytotoxic T cells are a vital component of the immune system. They have the remarkable ability to identify and target antigens on infected or abnormal cells. These antigens often originate from intracellular pathogens such as viruses or abnormal proteins cancer cells produce.
Immunological surveillance is the ability of immune cells to monitor and eliminate infected cells with intracellular pathogens, neoplastically transformed cells, and cells with non-self antigens. Cytotoxic T cells and NK...