在Litsea glaucescens中,Kuth具有针对Listeria monocytogenes的杀菌活性
Carlos David Gress-Antonio1, Nallely Rivero-Perez1, Silvia Marquina-Bahena2
1Área Académica de Medicina Veterinaria y Zootecnia, Universidad Autónoma del Estado de Hidalgo, Tulancingo de Bravo, Hidalgo, Mexico.
PeerJ
|December 6, 2023
概括
利塞亚青 (Litsea glaucencens Kuth) 叶子对Listeria monocytogenes具有抗菌性质. 它们的活性化合物pinocembrin具有杀菌活性,具有治疗李斯特菌病的潜力.
科学领域:
- 植物化学 植物化学
- 微生物学 微生物学
- 药理学 药理学是指药理学的学科.
背景情况:
- 传统上,Litsea glaucencens Kuth在墨西哥和食品调味品中使用.
- 它的叶子具有抗菌性质,特别是对Listeria monocytogenes,一种引起菌病的食物传播病原体.
- 在易受感染的个体中,病可能是致命的.
研究的目的:
- 调查Litsea glaucencens Kuth树叶提取物对Listeria monocytogenes的抗菌活性.
- 确定负责这种活动的特定生物活性成分.
主要方法:
- 使用不同极性的溶剂 (n-hexane,二甲,乙烯酸,甲醇) 提取叶子.
- 使用阿加扩散和微稀释方法评估抗菌活性,以确定最小抑制度 (MIC) 和最小杀菌度 (MBC).
- 活性化合物通过列色谱法进行净化,并使用NMR光谱学阐明结构.
主要成果:
- 甲醇提取物 (LgMeOH) 显示出显著的抗菌活性.
- 活性化合物被确定为5,7-二二黄 (pinocembrin).
- 皮诺坎布林显示出对L. monocytogenes的杀菌活性,MIC和MBC值为0.68 mg/mL,MBC/MIC比率为1.0.
结论:
- 利塞亚绿 (Litsea glaucencens Kuth) 叶及其代谢物皮诺森布林具有针对Listeria monocytogenes的杀菌活性.
- 这些发现表明,在治疗李斯特菌病方面,有潜在的治疗应用.
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