相关实验视频
Updated: Jul 9, 2025

09:23
Lateral Root Inducible System in Arabidopsis and Maize
Published on: January 14, 2016
13.8K
与auxin相关基因的长非编码RNA介导的表观遗传调节控制了Arabidopsis中的避阴综合征
María Florencia Mammarella1, Leandro Lucero1, Nosheen Hussain2
1Instituto de Agrobiotecnología del Litoral, CONICET, Universidad Nacional del Litoral, Santa Fe, Argentina.
The EMBO journal
|December 6, 2023
概括
长非编码RNAAPOLO通过调节BRANCHED1表达和染色质循环来调节Arabidopsis的阴影回避. 外源的APOLO RNA应用迅速增加了辅酶信号,表明可持续农业的潜力.
科学领域:
- 植物分子生物学 植物分子生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 植物生理学 植物生理学
背景情况:
- 长非编码RNAs (lncRNAs) 在基因调节中起着至关重要的作用.
- 避免阴影综合征 (SAS) 是植物对竞争光线的关键反应.
- 已知 lncRNA APOLO 与染色质相互作用,并调节辅酶平衡.
研究的目的:
- 为了研究APOLO在调节沙龙的避阴综合征中的作用.
- 阐明APOLO在SAS期间调节基因表达的分子机制.
- 探索外源APOLORNA作为农业工具的潜力.
主要方法:
- 在深红光条件下分析APOLO和BRC1表达.
- 染色体构造捕获技术用于研究BRC1位点的R循环形成和染色体循环.
- 对植物组织外源应用APOLO RNA,测量辅酶信号传递.
- 植物分枝和叶子低的表型分析.
主要成果:
- 在对远红光的反应中,APOLO表达与BRANCHED1 (BRC1) 表达具有反相关性.
- APOLO积累微调BRC1促进体的抑制色素环形成,导致转录抑制和增加分支.
- 外源的APOLO RNA应用迅速增强了辅酶信号传递,并影响了植物对远红光的反应.
- 通过其调节与auxin相关的基因,APOLO在叶子下沉中的作用得到了证实.
结论:
- 在阿波罗的例子中, lncRNAs 充当了 Arabidopsis 中避阴综合征的关键协调者.
- 阿波罗调节染色体构造,以调节关键的发育途径,如树枝的分支.
- 对外应用APOLO RNA证明了其作为调节植物环境相互作用的生物活性分子的潜力,并为可持续农业提供了一种新方法.
相关概念视频
Cell Signaling in Plants
5.6K
Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
5.6K
lncRNA - Long Non-coding RNAs
8.6K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
8.6K
Photoreceptors and Plant Responses to Light
20.4K
Light plays a significant role in regulating the growth and development of plants. In addition to providing energy for photosynthesis, light provides other important cues to regulate a range of developmental and physiological responses in plants.
20.4K
Epistasis Analysis
5.0K
Although Mendel chose seven unrelated traits in peas to study gene segregation, most traits involve multiple gene interactions that create a spectrum of phenotypes. When the interaction of various genes or alleles at different locations influences a phenotype, this is called epistasis. Epistasis often involves one gene masking or interfering with the expression of another (antagonistic epistasis). Epistasis often occurs when different genes are part of the same biochemical pathway. The...
5.0K
Transcription Attenuation in Prokaryotes
15.3K
Transcriptional attenuation occurs when RNA transcription is prematurely terminated due to the formation of a terminator mRNA hairpin structure. Bacteria use these hairpins to regulate the transcription process and control the synthesis of several amino acids including histidine, lysine, threonine, and phenylalanine. Transcription attenuation takes place in the non-coding regions of mRNA.
There are several different mechanisms used to attenuate transcription. In ribosome mediated...
There are several different mechanisms used to attenuate transcription. In ribosome mediated...
15.3K
Epigenetic Regulation
31.1K
Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
31.1K

