在Haemophilus influenzae生物群aegyptius的相变控制了多种蛋白质的表达
Greg Tram1, Freda E-C Jen1, Zachary N Phillips1
1Institute for Glycomics, Griffith University , Gold Coast, Queensland, Australia.
Microbiology spectrum
|December 6, 2023
概括
在Haemophilus influenzae生物群 aegyptius的相变改变了DNA甲基化和蛋白质表达. 这种由ModA相变介导的表观遗传切换会影响基因调节,但不会影响巴西紫热病株的血清耐药性.
科学领域:
- 微生物学 微生物学
- 细菌病原体的产生
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
背景情况:
- 海莫菲勒斯流感生物组埃吉普蒂乌斯会引起巴西紫热 (BPF),这是一个严重的儿童疾病.
- 阶段变异,一个可逆的基因表达开关,对于细菌的适应和毒性至关重要.
- 通过DNA甲基转移酶调节的相变异,控制细菌中的基因表达.
研究的目的:
- 在H. influenzae生物群 aegyptius中对ModA阶段变异基因 (ModA13和ModA16) 的表征.
- 为了研究相变对DNA甲基化和蛋白质表达的影响.
- 为了确定阶段变化是否影响BPF相关菌株的血清耐药性.
主要方法:
- 在BPF和非BPF菌株中对ModA等位基因的基因组分析.
- 对DNA甲基化模式的评估.
- 蛋白质组分析以检测蛋白质表达的变化.
- 血清耐药性测试. 血清耐药性测试.
主要成果:
- 确定了两个不同的ModA相变异基因,ModA13 (非BPF) 和ModA16 (BPF).
- ModA13和ModA16的相位变化诱导了全基因组DNA甲基化变化.
- 改变的DNA甲基化导致蛋白质表达特征的变化.
- 血清耐药性没有受到ModA阶段变化的影响.
结论:
- ModA阶段变异在H. influenzae生物群 aegyptius中起到表观遗传开关的作用,影响基因表达.
- 不同的ModA基因基因可能会导致H. influenzae生物组egyptius的独特病变型.
- ModA的相变似乎不是这些细菌血清耐药性的关键因素.
相关概念视频
Leaky Scanning
5.1K
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA. Marilyn Kozak discovered that the sequence RCCAUGG (where R...
5.1K
Prokaryotic Transcriptional Activators and Repressors
21.1K
The organization of prokaryotic genes in their genome is notably different from that of eukaryotes. Prokaryotic genes are organized, such that the genes for proteins involved in the same biochemical process or function are located together in groups. This group of genes, along with their regulatory elements, are collectively known as an operon. The functional genes in an operon are transcribed together to give a single strand of mRNA known as polycistronic mRNA.
Transcription of prokaryotic...
Transcription of prokaryotic...
21.1K
Regulation of Expression Occurs at Multiple Steps
22.7K
Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
22.7K
Bacterial RNA Polymerase
29.6K
Unlike eukaryotes, bacteria use a single RNA Polymerase (RNAP) to transcribe all genes. The different subunits of bacterial RNAPhave distinct functions. The multisubunit structure of the bacterial RNAP helps the enzyme to maintain catalytic function, facilitate assembly, interact with DNA and RNA, and self-regulate its activity.
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...
29.6K
Regulation of Expression at Multiple Steps
917
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
917
Protein Complexes with Interchangeable Parts
2.5K
Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order...
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order...
2.5K


