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相关概念视频

Labeling DNA Probes03:31

Labeling DNA Probes

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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
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相关实验视频

Updated: Jul 9, 2025

Fluorescence-microscopy Screening and Next-generation Sequencing: Useful Tools for the Identification of Genes Involved in Organelle Integrity
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使用SIMBA系统进行单基因组位点标记和操纵.

Yanwei Chen1,2, Ziliang Huang3, Qin Peng1

  • 1Institute of Systems and Physical Biology, Shenzhen Bay Laboratory, Shenzhen, China.

Current protocols
|December 6, 2023
PubMed
概括

SIMBA系统使用CRISPR技术,同时对活细胞中的基因组位点进行成像和操纵. 这种创新工具有助于研究基因组组织和基因表达调节.

关键词:
这就是CRISPR/Cas9的作用.这就是HP1α.染色素建模 染色素建模活细胞成像技术使用.位置标记标签的位置标签.

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科学领域:

  • 分子生物学分子生物学
  • 遗传学 是一个遗传学.
  • 细胞生物学 细胞生物学

背景情况:

  • 基于CRISPR的技术提供了精确的基因组定位.
  • 实时可视化和操纵基因组位置对于理解细胞过程至关重要.
  • 现有的方法可能缺乏同时成像和操纵的能力.

研究的目的:

  • 引入SIMBA (生物分子组件对基因组位点的同时成像和操纵) 系统.
  • 展示SIMBA对基因组位置的同时可视化和操纵的能力.
  • 为在各种细胞生物学应用中利用SIMBA提供协议.

主要方法:

  • SIMBA系统使用基于CRISPR的方法,使用dCas9-SunTag进行准.
  • FRB-mCherry-HP1α融合蛋白在目标位置促进染色质的凝聚.
  • 一种scFv-FKBP桥梁蛋白将组件连接起来,以有效地招募HP1α.

主要成果:

  • SIMBA 能够精确地可视化特定的基因组部位.
  • 该系统允许在目标位置缩染色素.
  • SIMBA促进了对基因组组织,基因表达和染色质动态的研究.

结论:

  • SIMBA系统是一个创新的工具,可以同时对基因组位置进行成像和操纵.
  • 这种技术为基因组组织和基因调节的研究开辟了新的途径.
  • SIMBA为活细胞成像和操纵研究提供了一个强大的平台.