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在口腔状细胞癌中,FBXW7通过MCL1影响自

Yang Sun¹, Wei Nie², Bo Qiu¹

¹Dental Clinic, Cangzhou Central Hospital, Cangzhou, China.

|December 6, 2023

概括

F-box/WD重复含有蛋白7 (FBXW7) 通过减少MCL-1和增强自抑制口腔癌. 这一发现为口腔状细胞癌 (OSCC) 治疗提供了潜在的治疗标.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
细胞过程细胞过程

背景情况:

口腔癌,特别是口腔状细胞癌 (OSCC),具有显著的发病率和死亡率风险.
对OSCC有效的治疗策略仍然是一个未满足的临床需求.
瘤抑制基因F-box/WD重复含有蛋白7 (FBXW7) 正在研究其在OSCC中的作用.

研究的目的:

调查FBXW7在自中的调节作用.
为了确定FBXW7是否可以改善口腔状细胞癌的预后.
阐明FBXW7,MCL-1和OSCC中的自之间的关系.

主要方法:

定量实时PCR (qRT-PCR) 用于测量OSCC和正常组织中FBXW7和MCL-1的mRNA水平.
用于在OSCC细胞系中过度表达FBXW7和MCL-1的lentiviral载体.
西方斑分析用于评估蛋白质表达水平.

主要成果:

在OSCC组织中,FBXW7mRNA和蛋白质水平下降,而MCL-1水平增加.
在OSCC患者中,自与蛋白质mRNA水平有所降低.
过度表达FBXW7导致MCL-1表达减少和自蛋白水平增加 (贝克林1,ATG7,LC3).

结论:

在口腔状细胞癌中,FBXW7在调节自中起着至关重要的作用.
该机制涉及FBXW7介导的MCL-1调节,这反过来又影响了自.
针对FBXW7可能是OSCC的新疗法.

关键词:

FBXW7FBXW7FFBXW7FF FBXW7FFF FBXW7FF FBXW7F 在MCL1中,MCL1是MCL1的第一个类型.自自是自的过程.口腔状细胞癌的癌症.

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