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外源性黑色素促进葡萄苗的寒冷耐受性:生理学,转录基因和功能证据

Junduo Li^1,2,3, Kai Lv^1,2,3, Jieping Wu^1,2,3

¹College of Enology and Horticulture, Ningxia University, Yinchuan 750021, Ningxia, China.

Journal of agricultural and food chemistry

|December 6, 2023

概括

黑色素 (MEL) 通过减少细胞损伤和保护叶绿体,增强葡萄的耐寒性. 它还调节植物激素水平,并激活特定的基因,如VvHSFA6b,以提高应激反应.

科学领域:

植物生理学植物生理学
分子生物学分子生物学
生物化学生物化学

背景情况:

黑素 (MEL) 是一种已知的抗氧化剂,可以改善植物的应激耐受性.
葡萄中MEL调节寒冷信号通路的具体机制尚未完全理解.

研究的目的:

研究用外源MEL治疗的葡萄苗中的生理和转录组变化.
为了确定MEL在葡萄寒冷耐受性的保护作用.

主要方法:

葡萄苗进行了外源MEL (150μM) 的处理.
生理学评估包括反应性氧物种 (ROS) 水平和叶绿体完整性.
进行了转录基因分析,以确定差异表达的基因.
对候选基因 (例如VvHSFA6b) 进行了功能分析.

主要成果:

MEL治疗减少了寒冷诱导的细胞损伤,ROS积累,并抑制了素降解.
外源MEL改变了内源激素水平,增加了ABA,auxin和cytokinin,同时减少了gibberellin.
转录组分析发现了776个差异表达的转录.
过度表达VvHSFA6b通过激活斯酸 (JA) 合成,提高了葡萄中的寒冷耐受性.

结论:

外源MEL为葡萄苗提供了对抗寒冷压力的显著保护.
在寒冷条件下,MEL影响葡萄中的多种生理过程和激素信号通路.
VvHSFA6b在通过JA途径调解MEL的耐寒效应方面发挥着至关重要的作用.

关键词:

这就是VvHSFA6b.寒冷的压力是冷的压力.功能分析是一种功能分析.黑激素是什么意思黑激素是什么意思保护作用保护作用

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