基因组学分析Chilomastix和Retortamonas物种使用体外脱落的鞭毛体
Jun Suzuki1, Sanjib Kumar Sardar2, Ajanta Ghosal2
1Division of Clinical Microbiology, Department of Microbiology, Tokyo Metropolitan Institute of Public Health, Shinjuku-ku, Tokyo, Japan.
概括
这项研究成功诱导了Chilomastix mesnili和Retortamonas sp.的脱离. 来自和蒙古斯. 遗传学分析揭示了与人类和两动物菌株的遗传相似之处,这表明宿主养习惯会影响寄生虫类型.
科学领域:
- 寄生虫学的寄生虫学
- 分子生物学分子生物学
- 微生物学 微生物学
背景情况:
- 奇洛马斯蒂克斯·梅斯尼利和雷托塔莫纳斯种类 (Retortamonas sp. 它们是肠道鞭子.
- 了解它们的外位和遗传多样性对于宿主寄生虫相互作用研究至关重要.
研究的目的:
- 为奇洛马斯提克 (Chilomastix mesnili) 和雷托塔莫纳斯 (Retortamonas sp.) 建立一个体外脱离站协议. 来自非人类宿主.
- 使用18S rRNA基因对这些分离物进行遗传学分析.
- 调查潜在的宿主-寄生虫关系和传播动态.
主要方法:
- 在实验室中使用修改的Giardia肠道结肠病协议和富含H2S的罗宾逊介质与Desulfovibrio desulfuricans.
- 从脱落的鞭毛体中分离DNA.
- 基于18S rRNA基因序列的遗传学分析.
主要成果:
- 在化后2小时内引发了外置状态.
- 来自日本的两个Chilomastix mesnili分离物与一个人类分离物聚集在一起.
- 一个Retortamonas sp. 的时间. 从一个小的印度蒙古斯群集与两动物隔离.
结论:
- 18S rRNA基因序列的同质性和形态相似性表明,类和人类菌株之间存在密切的关系.
- 这种两类型的Retortamonas sp. 蒙古斯的感染突出了宿主养习惯在寄生虫传播中的潜在作用.
- 这项研究提供了这些鞭毛动物的遗传多样性和宿主特异性的见解.
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