对非传统酵母中的β-葡萄糖酶活性进行比较分析
Juan Carlos González-Hernández1, Juan David Ramírez-Conejo1, Yolanda Patricia García-Aguirre1
1Tecnológico Nacional de México/Instituto Tecnológico de Morelia, Av. Tecnológico 1500, Col. Lomas de Santiaguito, C.P. 58120, Morelia, Michoacán, México.
Anais da Academia Brasileira de Ciencias
|December 6, 2023
概括
这项研究强调了非传统酵母的潜力,如Pichia pastoris和Candida utilis,作为β-葡萄糖酶的生产者. 这些酵母表现出显著的活性,特别是与葡萄糖和糖,用于生物质转化和多降解中的应用.
科学领域:
- 生物化学 生物化学
- 微生物学 微生物学
- 酶学 是一种酶学.
背景情况:
- β-葡萄糖酶酶在素的酶性降解中发挥作用,包括素.
- 埃拉基坦宁是营养学化合物,在食品,制药和抗菌材料中具有潜在的应用.
- 非传统的酵母因其酶能力而被探索.
研究的目的:
- 为了评估非传统酵母中的β-葡萄糖酶活性,使用纤维素,葡萄糖和砂糖基质.
- 为了识别具有高β-葡萄糖酶生产潜力的酵母菌株.
- 探索这些酵母在生物质转化和多降解中的应用.
主要方法:
- 在各种酵母菌株 (Candida,Pichia,I. terricola,P. kluyvery) 中评估细胞外β-葡萄糖酶活性.
- 使用葡萄糖,糖糖和纤维素作为基质来诱导酶活性.
- 使用刚果红色方法对纤维素降解的定性评估.
主要成果:
- 坎迪达和皮奇亚菌株表现出显著的细胞外β-葡萄糖酶活性.
- 皮奇亚牧草在葡萄糖介质中表现出最大的活性 (0.1682±0.00 μmol/min mg蛋白质).
- 在纤维素 (0.1129±0.1349μmol/分钟毫克蛋白质) 和糖 (0.0657±0.0214μmol/分钟毫克蛋白质) 中,Candida utilis表现出高活性.
- I. terricola和P. kluyvery显示出显著的质量纤维素降解.
结论:
- 皮奇亚牧草和Candida utilis被确定为有前途的β-葡萄糖酶生产者.
- 这些酵母显示出在生物反应器过程中与复杂的碳来源一起使用的潜力.
- 应用包括生物质转化,生物燃料生产和高效的多降解.
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