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Updated: Jul 9, 2025

Embryo Microinjection and Knockout Mutant Identification of CRISPR/Cas9 Genome-Edited Helicoverpa Armigera Hübner
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Published on: July 1, 2021

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需要GARP和EARP才能有效地复制BoHV-1,这是由全基因组的CRISPR淘汰屏幕识别出来的

Wenfang S Tan1, Enguang Rong1, Inga Dry1

  • 1Division of Infection and Immunity, University of Edinburgh, Edinburgh, Scotland, United Kingdom.

PLoS pathogens
|December 6, 2023
PubMed
概括

一个新的牛CRISPR淘汰库有助于发现影响牛疹病毒1型 (BoHV-1) 复制的基因. 关键发现揭示了GARP和EARP复合体在病毒包装和组装中的重要作用.

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Homologous Recombination02:31

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
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