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针对牛肉病的血清学测试的准确性:系统性审查和元分析

Rafaella Silva Andrade¹, Marina Martins de Oliveira¹, Júlio Sílvio de Sousa Bueno Filho²

¹Departamento de Medicina Veterinária, Faculdade de Zootecnia e Medicina Veterinária, Universidade Federal de Lavras, Lavras, Minas Gerais, Brazil.

Preventive veterinary medicine

|December 6, 2023

概括

这项研究评估了针对牛牛病的血清学测试,发现间接酶免疫测试 (iELISA) 和光极化测试 (FPA) 提供了最佳的诊断准确性. 当包括病例控制研究时,注意到过度估计了测试性能,影响了疾病控制计划.

科学领域:

兽医医学兽医医学兽医医学
免疫学免疫学免疫学
流行病学流行病学

背景情况:

直接诊断牛血病的方法有局限性,使得血清学测试对于疾病诊断至关重要.
缺乏对全球牛肉牛病控制计划测试的诊断灵敏度和特异性的综合元分析.

研究的目的:

系统地审查和元分析用于诊断牛肉病的血清学测试的诊断灵敏度 (DSe) 和诊断特异性 (DSp).
估计针对牛牛肉病的单个血清学测试的整体准确性.

主要方法:

来自多个数据库 (CABI,Cochrane图书馆,PubMed/MEDLINE,SciELO,Scopus,Web of Science) 的研究的系统审查和元分析.
包括71项用于系统审查的研究和65项用于元分析的研究,考虑了178种测试和11种不同的血清学测试.
根据研究设计分层分析 (非病例控制与包括病例控制在内的所有研究),以评估诊断准确性.

主要成果:

使用细菌悬浮抗原的间接酶免疫测试 (iELISA) 显示了最高的DSe (96.5%),而2-mercaptoethanol (2ME) 测试显示了最低的 (85.0%).
光极化试验 (FPA) 显示了最高的DSp (99.7%),而蛋白质度光免疫试验 (PCFIA) 则是最低的 (78.5%).
包括病例控制研究导致大多数测试的DSe和DSp被高估,影响了成本效益评估.

结论:

光极化试验 (FPA) 显示了牛牛病的最佳整体诊断准确性.
iELISA (BS) 和FPA因其高性能和易于使用而被推用于牛牛病控制计划.
在元分析中使用病例控制研究数据时,建议对测试性能指标的潜在高估保持谨慎.

关键词:

牛类牛类牛类牛类牛类牛布鲁塞拉流产 (Brucella abortus) 是一种流产.诊断诊断诊断诊断诊断诊断的敏感性诊断的敏感性诊断的特异性诊断的特异性

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