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在东印度患者群体中前列腺癌发生期间DNA表观遗传调节器的调节失调:预后影响
Anwesha Banerjee1, Abhishek Bardhan1, Purandar Sarkar2
1Department of Life Sciences, Presidency University, Kolkata, West Bengal, India.
Pathology, research and practice
|December 6, 2023
概括
前列腺癌的表观遗传变化涉及DNA甲基转移酶 (DNMTs) 和脱甲基酶 (TETs). DNMT1的升调和TET1/TET2的沉默与前列腺癌的发展和进展有关,TET促进剂甲基化表明预后不佳.
科学领域:
- 在瘤学瘤学.
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 分子生物学分子生物学
背景情况:
- 表观遗传改变在前列腺癌的发病过程中起着至关重要的作用.
- DNA甲基转移酶 (DNMTs) 和脱甲基酶 (TETs) 是DNA甲基化的主要调节者.
- 了解它们的相互作用对于阐明前列腺瘤发生至关重要.
研究的目的:
- 分析前列腺癌发育期间DNMTs和TETs的失调.
- 研究前列腺瘤发生过程中DNA甲基化和脱甲基化途径之间的相互作用.
- 为了将表观遗传变化与前列腺癌的临床病理特征相关联.
主要方法:
- 在正常前列腺,BPH和PCa组织中分析DNMTs和TETs的促进物甲基化和RNA/蛋白质表达.
- 基因组中的5-基甲基细胞素 (5hmC) 水平的量化.
- 分子数据与临床病理学参数的相关性.
主要成果:
- DNMT1促进物甲基化下降,而TET1和TET2促进物甲基化从正常增加到PCa.
- DNMT1过度表达,TET1/TET2在PCa下调,与促进物甲基化相关.
- 在PCa中降低的5hmC水平与DNMT1负相关,与TET1/TET2.2正相关.
- 甲基化TET1/TET2促进剂与先进的临床病理特征和骨转移风险有关.
结论:
- 在前列腺癌的发展过程中,DNMT1的上调和TET1/TET2的表观遗传沉默发生.
- DNMTs和TETs的表观遗传失调有助于前列腺瘤发生.
- 在前列腺癌中,TET1和TET2促进剂甲基化具有显著的预后价值.
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