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新型NIR光探针用于在生物系统中检测化离子

Jian Zhang¹, Yu Nan², Hushan Wang¹

¹Department of Anesthesiology, First Hospital of Jilin University, Changchun 130021, Jilin, China.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy

|December 6, 2023

概括

研究人员开发了一种新的光探针NR-ClO,用于在生物环境中检测酸盐离子 (ClO-). 这种敏感和选择性的探测器可以在复杂的环境中实时监测,为疾病研究显示出前景.

科学领域:

化学生物学化学生物学
生物医学工程生物医学工程
分析化学分析化学

背景情况:

酸盐离子 (ClO-) 是一种反应性氧物种,参与各种生理和病理过程.
准确检测ClO-对于了解细胞功能和疾病机制至关重要.
现有的检测方法往往缺乏生物系统的灵敏度,选择性或实时监测能力.

研究的目的:

合成和描述一种新的光探针NR-ClO,用于敏感和选择性检测化离子.
评估探头在复杂的生物环境中的性能,包括体外和体外模型.
建立NR-ClO作为生物研究中实时ClO监测的可靠工具.

主要方法:

在尼罗河红色和二甲基碳化物之间通过核替代合成NR-ClO探针.
探测器光物理性质的光谱表征及其对化离子的反应.
在体外使用HepG2细胞和体内使用风湿性关节炎小鼠模型验证探针的疗效.

主要成果:

该NR-ClO探针对化离子具有很高的灵敏度和选择性,其低检测极限为75nM,线性范围广 (0.1-200μM).
该探测器成功地在HepG2细胞和风湿性关节炎小鼠模型中实时监测了酸盐离子.
在测试的细胞和动物模型中,没有观察到探针的显著细胞毒性.

结论:

新型NR-ClO光探针是一种高度敏感和选择性的工具,用于在生物系统中检测化离子.
NR-ClO 能够在体外和体内环境中可靠地实时监测酸盐离子.
这种探测器具有很大的潜力,可以促进与氧化压力相关的疾病和生物过程的研究.

关键词:

细胞成像细胞成像光探测器探测器可以发光.过化离子过酸离子尼罗河红色尼罗河红色类风湿性关节炎类风湿性关节炎类风湿性关节炎

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