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Updated: Jul 9, 2025

Collection and Extraction of Saliva DNA for Next Generation Sequencing
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对口腔细胞学样本的不同DNA保存溶液的比较

Júlia Silveira Nunes1, Luisa Natalia Pimentel-Vera2, Sabrina Barcelos Silva1

  • 1Department of Oral Pathology, Federal University of Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil.

Archives of oral biology
|December 6, 2023
PubMed
概括

甲基硫氧化物二-EDTA和NaCl (DESS) 缓冲剂有效地在4°C下保存细胞DNA长达3个月. 这项研究比较了DNA保存解决方案,发现DESS在长期存储和分析方面优越.

关键词:
它们是DNA DNA DNA DNA.DNA 保存解决方案 解决方案分子分析分子分析口腔细胞学是指口腔细胞学.口腔癌是一种口腔癌.

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DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
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