在高温症期间,哺乳乳奶牛的结肠中细胞和线粒体适应机制
Mehdi Eslamizad1, Dirk Albrecht2, Björn Kuhla1
1Research Institute for Farm Animal Biology (FBN), Institute of Nutritional Physiology "Oskar Kellner," 18196 Dummerstorf, Germany.
Journal of dairy science
|December 6, 2023
概括
奶牛的热应激会在结肠中引发显著的变化,包括炎症和障碍功能受损. 这项研究揭示了细胞适应,旨在减少细胞损伤,并在暴露于热量时保持能量水平.
科学领域:
- 动物科学动物科学
- 生理学 生理学 生理学
- 分子生物学分子生物学
背景情况:
- 热应激对动物健康和生产力产生负面影响,特别是在奶牛中.
- 小肠对热应激的反应已被记录,但大肠的机制仍然不太了解.
- 了解牛结肠对高热的反应对于动物福利和农业效率至关重要.
研究的目的:
- 为了研究在恒定的热应力下,牛结肠中的分子和细胞变化.
- 为了确定乳牛经历热应激的结肠粘膜中的蛋白质变化.
- 阐明牛肠在暴露于热量的过程中的适应机制和潜在的功能障碍.
主要方法:
- 来自荷尔斯坦乳牛的结肠粘膜样本的蛋白质组分析.
- 在热中性条件下比较奶牛,热应激,在热中性条件下配对养.
- 分析生理学数据,包括直肠温度,牛奶产量和料/水摄入量.
主要成果:
- 热应激的奶牛表现出明显更高的直肠温度.
- 蛋白质组分析揭示了与结肠屏障功能,炎症和热冲击蛋白相关的激活通路.
- 代谢调整包括氨基酸代谢的变化,蛋白质合成的减少以及三碳酸循环和线粒体电子运输链的改变.
结论:
- 热应激诱导了牛结肠中显著的细胞,细胞外和线粒体适应.
- 这些适应似乎减轻了细胞损伤,并维持了能量恒温.
- 这些发现表明后肠屏障功能障碍的可能性,导致热应激乳牛的"漏"结肠.
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