连续培养微藻用于废水处理 - - 制定白天和夜晚的工艺策略
Franziska Ortiz Tena1, Victoria Bickel1, Christian Steinweg1
1Karlsruhe Institute of Technology (KIT), Institute of Process Engineering in Life Sciences, Bioprocess Engineering, Fritz-Haber-Weg 2, 76131 Karlsruhe, Germany.
The Science of the total environment
|December 6, 2023
概括
微藻可以通过回收营养和储存生物质,持续白天和晚上处理废水. 这项研究表明,Chlorella vulgaris可以达到完全的营养吸收,并产生有价值的生物质用于废水净化.
科学领域:
- 环境生物技术环境生物技术
- 微藻污水处理 微藻污水处理
- 营养物质回收利用 营养物质回收利用
背景情况:
- 传统的废水处理 (WWT) 与直接的营养物质回收作斗争.
- 微藻有效地从废水中吸收和.
- 太阳光依赖性限制了连续的微藻 WWT 过程.
研究的目的:
- 调查持续培养的Chlorella vulgaris在光明和黑暗周期中的代谢活动.
- 确定合成废水在夜间吸收营养的能力.
- 定义连续微藻 WWT 的最佳运行条件.
主要方法:
- 在不同的稀释速率 (0.0-1.0d-1) 下,连续种植Chlorella vulgaris.
- 对代谢活动,营养吸收和生物质组成 (碳水化合物,脂质,蛋白质,颜料) 的分析.
- 增加的实验证实了夜间的营养同化.
主要成果:
- 观察到的营养物质的完整吸收率高达0.5d-1.1的稀释率.
- 微藻在低稀释率下积累了高达70%的储存成分 (碳水化合物,脂质),在黑暗中将粉转化为脂质.
- 在最佳条件下 (0.5d-1),生物质表现出高蛋白质 (57%DBM) 和色素 (6.9%DBM) 含量,夜间蛋白质由储存降解而积累.
结论:
- 使用微藻的连续废水处理是可行的24/7没有人工光线.
- 最佳运行包括细胞回收和储存富含碳水化合物的生物质,随后是夜间蛋白质生产.
- 该过程能够有效地去除营养物质,并产生有价值的,富含蛋白质的生物质,用于进一步利用.
相关概念视频
01:30Microbial Wastewater Treatment
Microbial communities in aquatic ecosystems play a key role in the natural breakdown of contaminants introduced through domestic and industrial effluents. Acting as biological catalysts, these microbes change and mineralize a wide range of organic and inorganic pollutants under different redox conditions.In oxygen-rich surface waters, aerobic heterotrophs lead organic matter breakdown, using oxygen as the terminal electron acceptor to efficiently oxidize substrates to carbon dioxide and water.
01:29Bioreactor Design and Operational System
Bioreactors are engineered vessels designed to cultivate microorganisms under controlled conditions for industrial bioprocessing. They maintain sterility and allow precise regulation of pH, temperature, oxygen, and nutrient levels to optimize microbial growth and metabolite production. Bioreactors range from small laboratory units of 1 liter to industrial systems holding up to 500,000 liters, though only about 75% of their volume is actively used for fermentation. The remaining headspace...
01:18Bioreactor Controls-II
In aerobic fermentations, oxygen is vital for microbial growth and metabolite production. Since air comprises only about 20% oxygen and the gas is poorly soluble in water—just 9 ppm at 20°C—supplying sufficient oxygen becomes a critical challenge, especially in high-demand processes like yeast growth or citric acid production. Even a fully saturated broth may offer only a few seconds of oxygen availability.To address this, sterile or scrubbed air is introduced into the fermentor via a sparger...
01:27Production of Alcohol
Continuous fermentation is a key strategy in industrial ethanol production, particularly when efficiency, scalability, and high yields are essential. This approach allows for uninterrupted operation and optimized resource utilization. The primary feedstock, corn starch, undergoes enzymatic hydrolysis facilitated by α-amylase and glucoamylase. These enzymes break down the starch into fermentable sugars such as glucose, which are readily assimilated by fermentative microorganisms.Fermentation...
01:30Biological Treatment of Effluent and Waste Water
Biological wastewater treatment relies on the metabolic activity of microorganisms to remove pollutants from sewage. In modern treatment systems, this process is organized into sequential stages that progressively reduce solid material, dissolved organic matter, and microbial contamination. Each stage plays a distinct role in improving water quality and preparing the effluent for safe discharge or reuse.Primary and Secondary TreatmentPrimary treatment is a physical process that removes large...
01:25Biofuels
The microbial conversion of organic matter into biofuels holds potential as a renewable energy source. Among biofuel sources, microalgae are recognized as a highly efficient and adaptable feedstock for biodiesel production, owing to their rapid biomass accumulation, elevated lipid productivity, and capacity to proliferate in diverse aquatic systems, including freshwater, marine, and wastewater habitats. Unlike terrestrial crops, microalgae do not compete for land and can achieve significantly...