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光碳点的双重功能化通过环氧和氨基来进行β-glucuronidase的视觉检测和生物成像

Chenhui Yin¹, Meng Wu¹, Qijun Sun¹

¹College of Chemistry, Chemical Engineering and Resource Utilization, Key Laboratory of Forest Plant Ecology, Northeast Forestry University, 26 Hexing Road, Harbin, 150040, China.

Analytica chimica acta

|December 6, 2023

概括

一种新方法使用功能化碳点 (β-CD-SiCD) 来检测β-葡萄糖酶活性. 这种灵敏,快速和低能耗的方法可以使用智能手机在现场进行检测.

科学领域:

生物化学生物化学
材料科学材料科学材料科学
分析化学分析化学

背景情况:

β-glucuronidase是生物系统中重要的酶.
敏感和快速检测方法对于β-葡萄糖酶活性分析至关重要.
现有的方法可能需要复杂的仪器或耗时.

研究的目的:

开发一种灵敏,快速和简单的光检测方法来检测β-glucuronidase.
使用功能化碳点 (β-CD-SiCD) 作为光探针用于酶检测.
为了能够在现场和基于智能手机的酶活性量化.

主要方法:

在室温下通过in situ自聚合合成β-CD-SiCDs.
使用4-尼托菲尼尔-β-D-葡萄化物作为基质生成p-尼托菲尼尔.
使用p-nitrophenol和β-CD-SiCD之间的宿主-客人识别导致光火.

主要成果:

β-CD-SiCD显示绿色光 (515 nm) 的量子产量为7.9%.
实现了0.560 U L-1的线性检测范围和0.14 U L-1的检测极限.
在血清样本和细胞成像中成功应用,证明了智能手机的现场检测能力.

结论:

建立了一个新的,灵敏的,快速的光传感平台来检测β-glucuronidase.
该方法具有成本效益,能源效率和用户友好性,适用于现场应用.
开发的平台显示了对生物和临床诊断的前景.

关键词:

碳点是指一个碳点.光传感器是一种光传感器.细胞内成像细胞内成像视觉检测视觉检测是一种视觉检测.这是一种β-Glucuronidase.

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