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亚酸介导的细胞溶液Ca2+调节了Ganoderma lucidum的三基积累

Meilin Cui¹, Yitao Zhao², Xiuhong Zhang²

¹College of Food Science, Shanxi Normal University, Taiyuan 030031, China. cuimeilin1988@163.com.

Journal of Zhejiang University. Science. B

|December 6, 2023

概括

亚酸 (ABA) 通过调节关键基因和激活信号通路,增强了Ganoderma lucidum中的甘三烯酸 (GT) 积累. 这个过程涉及抗氧化剂防御调节,改善的药用特性.

科学领域:

菌类学菌类学是指菌类学.
植物生理学植物生理学
生物化学生化学

背景情况:

甘光 (Ganoderma lucidum) 由于其食用和药用特性而受到重视,甘三聚类 (GTs) 是其主要生物活性化合物.
植物激素酸 (ABA) 影响真菌生长和新陈代谢,并与植物中的三烯调节有关.

研究的目的:

为了研究ABA在调节Ganoderma lucidum中GT生物合成中的作用.
阐明信号通路,包括 (Ca2+) 和抗氧化剂反应,参与ABA介导的GT积累.

主要方法:

用ABA对光虫进行治疗,并分析GT含量.
在三二生物合成途径 (sqs, hmgr, ls) 中关键酶的基因表达分析.
研究细胞质Ca2+水平,Ca2+通道活性以及Ca2+捐赠者/抑制剂的影响.
评估抗氧化酶活动 (SOD,APX) 和活性氧物种/谷氨水平.

主要成果:

在Ganoderma lucidum中,ABA治疗显著促进了GT积累.
ABA上调了参与GT生物合成的基因表达 (sqs, hmgr, ls).
ABA激活了细胞质Ca2+通道,并且证实Ca2+信号对于ABA介导的GT生物合成至关重要.
ABA调节了抗氧化防御,增加了超氧化脱氧化酶 (SOD) 和酸盐过氧化酶 (APX) 的活性,并增强了对氧化应激的抵抗力.

结论:

阿布西斯酸是Ganoderma lucidum中甘三烯酸生物合成的关键调节剂.
由ABA介导的GTs的增加涉及特定生物合成基因和信号通路的调节.
ABA增强了的抗氧化能力,有助于其整体的药用潜力.

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