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目标驱动年状DNA步行器与电化学发光量子点相结合,用于APE1生物分析

Ying-Huan Li¹, Mei-Ling Zhao¹, Pu Zhang¹

¹Key Laboratory of Luminescence Analysis and Molecular Sensing (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, P. R. China.

Analytical chemistry

|December 6, 2023

概括

这项研究开发了一种稳定,目标驱动的环状DNA步行器,用于检测apurinic/apyrimidinic内核酶1 (APE1). 增强的量子点 (SiQD) 在临床样本中提供灵敏且可靠的电化学发光 (ECL) 检测.

科学领域:

纳米技术纳米技术
生物技术是生物技术.
分析化学分析化学

背景情况:

DNA步行者是有前途的纳米结构,但由于核酶,生物样本的不稳定性.
目前用于检测像APE1这样的酶的方法在灵敏度和可靠性方面面临挑战.

研究的目的:

开发一种抗核酶的环状DNA步行器,用于敏感且可靠地检测apurinic/apyrimidinic内核酶1 (APE1).
为了提高量子点 (SiQDs) 的稳定性和电化学发光 (ECL) 性能,用于生物传感应用.

主要方法:

通过透析净化,制备具有增强ECL稳定的量子点 (SiQD).
针对APE1检测的目标驱动环状DNA步行器的设计和合成.
固定DNA步行器和火探头在SiQD修改的电极上,用于ECL读出.

主要成果:

透析净化显著改善了SiQD ECL信号稳定性 (RSD的16.59倍改善).
开发的ECL生物传感器证明了APE1检测的广泛线性范围 (1.0 × 10-13 到 1.0 × 10-7 U·μL-1).
为APE1.1实现了1.766 × 10-14 U·μL-1的低检测极限 (LOD).

结论:

新型环状DNA步行器表现出增强的核酶抵抗力,可用于可靠的临床样本检测.
优化的SiQD为ECL生物传感提供了一个强大的平台,扩展SiQD应用.
这项工作提出了开发稳定的DNA步行器和高性能ECL生物传感器的新策略.

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