通过TLR4/MyD88/NF-κB途径减轻牛皮表皮细胞中LPS诱导的炎症
Panpan Tan1, Jiaqi Yang1, Fanxuan Yi1
1College of Veterinary Medicine, Northwest A&F University, Yangling, 712100, Shaanxi, China.
Biological trace element research
|December 6, 2023
概括
(Sr) 通过抑制TLR4/MyD88/NF-κB通路来减少牛细胞的炎症. 这一发现为乳牛养殖中亚急性反动物酸化症 (SARA) 提供了潜在的治疗方法.
科学领域:
- 兽医医学 兽医医学 兽医医学
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
背景情况:
- 亚急性反虫酸性疾病 (SARA) 是反动物中普遍存在的代谢疾病,导致乳制品养殖业的经济损失很大.
- 斯特 (Sr) 已因其在骨代谢中的作用而闻名,并具有显著的抗炎性质.
研究的目的:
- 为了研究 (Sr) 对牛皮上皮细胞中脂聚糖 (LPS) 诱导的炎症的抗炎作用.
- 阐明Sr的作用背后的分子机制,特别关注TLR4/MyD88/NF-κB信号通路.
主要方法:
- 在牛皮上皮细胞中建立LPS诱导的炎症模型.
- 使用CCK-8试验评估细胞活力.
- 通过ELISA和实时PCR对促炎性细胞因子 (TNF-α,IL-1β,IL-6) 的量化.
- 分析TLR4/MyD88/NF-κB通路蛋白质和p-p65和p-IκB的光,使用西方涂抹和免疫光.
- 模拟Sr与途径相关蛋白质的分子对接.
主要成果:
- 在高度 (≥10 mmol/L) 的 (Sr) 治疗24小时后降低了细胞活力.
- Sr显著降低了促炎性细胞因子TNF-α,IL-1β和IL-6的产生.
- Sr降低了TLR4/MyD88/NF-κB通路中蛋白质的表达,并降低了p-p65和p-IκB的光.
- 用NF-κB抑制剂 (PDTC) 进行的分子对接和实验证实了Sr通过TLR4/MyD88/NF-κB通路的作用.
结论:
- 斯特伦 (Sr) 通过抑制TLR4/MyD88/NF-κB通路,在牛头上皮细胞中发挥抗炎作用.
- Sr减少了LPS诱导的促炎性细胞因子的产生,这表明其在牛酸性腐蚀的潜在治疗应用.
- 这些发现为在反动物中的SARA管理中利用Sr提供了科学依据.
更多相关视频
08:24Injections of Lipopolysaccharide into Mice to Mimic Entrance of Microbial-derived Products After Intestinal Barrier Breach
Published on: May 2, 2018
19.6K
05:52Author Spotlight: Induction of Experimental Endotoxemic Shock in Pigs for Studying Hemodynamic and Respiratory Failure
Published on: December 8, 2023
970
相关概念视频
Formation of Lipopolysaccharides
Lipopolysaccharides (LPS) are crucial components of the outer membrane of Gram-negative bacteria, serving both structural and functional roles. It contributes to membrane stability and protects bacteria from host immune responses. LPS is composed of three major regions—lipid A, a core oligosaccharide, and an O antigen. The biosynthesis and assembly of LPS involve a highly coordinated set of enzymatic reactions and transport mechanisms. Additionally, LPS is recognized as an endotoxin, triggering...
Clinical Significance of Antibiotic Resistance
Methicillin-resistant Staphylococcus aureus (MRSA) presents a critical public health threat, arising from its capacity to resist β-lactam antibiotics due to acquisition of the mecA gene within the staphylococcal cassette chromosome mec (SCCmec). This gene encodes penicillin-binding protein 2a (PBP2a), which impairs binding efficacy of methicillin and other β-lactams. MRSA has evolved into distinct clonal lineages impacting humans and animals alike, reinforcing its significance within the One...
Inflammatory Bowel Disease II: Ulcerative Colitis
Ulcerative colitis is a chronic inflammatory disorder of the colon characterized by continuous mucosal inflammation that typically begins in the rectum and extends proximally in a uniform pattern. Its pathogenesis involves a complex interplay of genetic predisposition, immune dysregulation, and environmental influences. These factors converge to impair the colon’s epithelial defenses and promote an exaggerated inflammatory response against luminal contents.Breakdown of the Mucosal BarrierA...
Inflammatory Bowel Disease III: Crohn's Disease
Crohn’s disease is a chronic, relapsing form of inflammatory bowel disease characterized by segmental, transmural inflammation that can affect any part of the gastrointestinal tract. Its pathogenesis arises from a combination of genetic susceptibility, environmental exposures, epithelial barrier dysfunction, and immune dysregulation. Together, these factors lead to an exaggerated immune response against components of the gut microbiome.Genetic and Environmental InfluencesMultiple genetic...
