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银纳米颗粒降低了肝癌中的Rap2A基因的调节

Sabahat Javid¹, Erum Dilshad²

¹Department of Bioinformatics and Biosciences, Faculty of Health and Life Sciences, Capital University of Science and Technology(CUST), Islamabad, 44000, Pakistan.

Scientific reports

|December 6, 2023

概括

银纳米颗粒有效地准肝癌细胞中的Rap2A,减少瘤生长并诱导亡. 这项研究提出了一种新的治疗方法来治疗肝癌.

科学领域:

在瘤学瘤学.
纳米技术纳米技术
药理学药理学是指药理学的学科.

背景情况:

肝癌是全球癌症死亡的主要原因之一.
作为一种Ras GTPase,Rap2A蛋白被认为是包括肝癌在内的各种瘤中的瘤基因.
向Rap2A为肝癌提供了一个潜在的治疗策略.

研究的目的:

从Artemisia carvifolia Buch提取物中合成和表征银纳米粒子 (AgNPs).
评估A. carvifolia提取物及其AgNP的抗肝癌疗效,特别是针对Rap2A基因.
为了研究A. carvifolia提取物和AgNP在肝癌细胞中的亡潜力.

主要方法:

使用UV-Vis光谱测量,SEM,FTIR,XRD和EDX合成和描述AgNP.
使用IC50值对HePG2肝癌细胞系进行细胞毒性评估.
评估Rap2A基因和蛋白质表达水平.
通过实时qPCR和ELISA对亡途径基因 (BAX,caspase 3,8,9) 和蛋白质的分析.

主要成果:

从A. carvifolia提取物合成的多面体AgNP (80±6nm) 的特征.
观察到显著的细胞毒性:对AgNPs的IC50为2.57μM,对HePG2细胞的提取物为11.57μM.
治疗细胞中的Rap2A基因和蛋白质表达减少.
在基因和蛋白质水平上,BAX,caspase 3,8和9的表达增加表明增强的亡.

结论:

A. carvifolia提取物介导的银纳米颗粒显示出显著的抗肝癌活性.
合成的AgNP有效地准并降低Rap2A的调节,Rap2A是肝癌中的关键瘤基因.
这项研究建立了一个新的,有效的,自然的治疗方法,使用A. carvifolia衍生的AgNP治疗肝癌.

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