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在光成像中确定最佳组织标记染料颜色用于病理评估,使用IRDye800CW

Takahito Kondo¹, Naoki Nishio², Jason S Park¹

¹Department of Otolaryngology-Head and Neck Surgery, Vanderbilt University Medical Center, Nashville, TN, USA.

Molecular imaging and biology

|December 6, 2023

概括

黄色,红色和色的组织标记染料 (TMD) 最适合用于IRDye800CW光成像. 避免蓝色和黑色的TMD,以确保准确的瘤样本评估. 这有助于优化光引导手术的结果.

科学领域:

医疗成像医学成像
在瘤学瘤学.
病理学病理学病理学

背景情况:

光导向手术利用瘤特异性抗体-染料合体,以增强各种癌症的可视化.
术内和外生光成像是外科瘤学和病理学中的关键工具.
选择用于ex vivo标记样品的油墨颜色可以干扰光染料信号,影响成像准确度.

研究的目的:

评估常用的组织标记染料 (TMD) 与IRDye800CW光成像的兼容性.
为了确定哪些墨水颜色最小化对IRDye800CW光的干扰,以准确评估瘤样本.

主要方法:

八种常见的组织标记染料被测试使用阿加罗斯幽灵与Panitumumab-IRDye800CW.
平均光强度使用封闭场 (Pearl Trilogy) 和开放场 (pde-neo II) 成像系统进行测量.
在体内小鼠异种移植模型的头部和部状细胞癌被用来验证发现.

主要成果:

在成像系统中,黄色,红色和色的TMD显示了IRDye800CW光的最高保留率 (86-91%).
蓝色和黑色TMD显著灭IRDye800CW光 (6-17%),蓝色显示最小的保留 (0.2%) 在体内.
紫色和绿色的TMD显示了中等水平的光保留.

结论:

在使用IRDye800CW光成像时,建议使用黄色,红色和色TMD标记瘤样本.
应避免使用蓝色和黑色的TMD,因为它们会严重干扰IRDye800CW光.
标记染料的正确选择对于在光导向程序中可靠的体外样本评估至关重要.

关键词:

光成像成像的使用方法光指导的手术是指导的手术.在IRDye800CW中使用.外科手术边缘评估的评估标记组织的染料.

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