单细胞基因组伴侣的模式指导瘤微环境的细胞间通信,这有助于乳腺癌转移
Jindong Xie1, Wei Deng1, Xinpei Deng1
1State Key Laboratory of Oncology in South China, Guangdong Provincial Clinical Research Center for Cancer, Sun Yat-sen University Cancer Center, 651 East Dongfeng Road, Guangzhou, 510060, China.
基因素陪伴体 (HCs) 在乳腺癌 (BC) 转移中引导瘤微环境 (TME) 细胞通信. 这项研究揭示了HCs.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 免疫学 免疫学 免疫学
背景情况:
- 基因组陪伴体 (HCs) 对于基因组稳定性和基因表达至关重要.
- 它们在乳腺癌 (BC) 转移中的瘤微环境 (TME) 内的具体作用仍然不清楚.
研究的目的:
- 为了研究HCs在BC大脑和肝脏转移的TME中的功能.
- 探索HCs,TME细胞通信,患者预后和免疫疗法反应之间的关联.
主要方法:
- 重新分析6个BC患者的单细胞RNA-seq数据,使用非负矩阵因子化 (NMF) 对36个HC进行分析.
- 在BC和免疫治疗队伍中对TME子集群的评估,用于预后和免疫反应评估.
- 使用跨井和伤口愈合试验对HSPA8的功能验证.
主要成果:
- 确定了与BC转移特征相关的独特的TME子集群 (纤维细胞,巨细胞,B细胞,T细胞).
- 证明HC介导的TME子集群,特别是涉及B细胞和巨细胞,可显著预测预后和免疫治疗反应.
- 揭示了HC相关的TME子集群和恶性细胞之间的广泛的细胞间通信,HSPA8缺乏抑制BC细胞迁移和入侵.
结论:
- 这项研究阐明了HCs在编排BC转移期间TME细胞间通信中的关键作用.
- 这些发现突出了HC作为潜在的治疗点,以改善BC患者的治疗结果和免疫疗法的有效性.
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