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在血管成熟过程中,Ang1/Tie2/VE-Cadherin信号调节DPSC

Y Zhang¹, S Lin¹, J Liu¹

¹Restorative Dental Sciences, Endodontics, Faculty of Dentistry, The University of Hong Kong, Hong Kong, China.

Journal of dental research

|December 7, 2023

概括

牙纸干细胞 (DPSCs) 增强血管的稳定性和寿命. 修改后的DPSCs,E-DPSCs和T-DPSCs,表现出改善的环状细胞类功能,通过调节关键信号通路来促进血管化.

科学领域:

再生医学是一种再生医学.
血管生物学血管生物学
干细胞疗法是一种干细胞疗法.

背景情况:

牙纸干细胞 (DPSC) 表现出类似细胞的功能,并支持内皮细胞 (EC) 网络.
通过DPSC衍生细胞稳定血管系统的精确机制仍然不完全理解.

研究的目的:

在体外和体内研究E-DPSCs和T-DPSCs的血管稳定功能.
阐明通过修改DPSCs增强血管稳定作用的分子机制.

主要方法:

在体外:3D培养球形发芽试验比较E-DPSCs和T-DPSCs.
在体内:在严重联合免疫缺陷 (SCID) 的小鼠模型中进行血管生成评估.
对信号通路的分子分析,包括Tie2,VE-cadherin和VEGFR2.

主要成果:

无论是E-DPSC还是T-DPSC,都表现出类似光滑肌肉细胞的特性,并抑制了EC发芽.
修改后的DPSC通过激活angiopoietin 1 (Ang1) / Tie2信号传递和调节VE-cadherin,稳定了血管.
在体内研究表明,在用E-DPSCs或T-DPSCs治疗的组中,血管 perfusion 和总血管数量增加.

结论:

E-DPSCs和T-DPSCs有效地稳定新形成的血管并增强输液.
Ang1/Tie2/VE-cadherin和VEGF/VEGFR2信号通路对于DPSC介导的血管稳定至关重要.

关键词:

牙纸是一种牙纸.人的带静脉内皮细胞周围细胞 (pericytes) 是一个细胞.干细胞是干细胞的组成部分.转化增长因子β1的转化增长因子β1 血管内皮生长因子受体2 血管内皮生长因子受体2

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