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在Artemisia物种中高度氧化的黄 - 结构修订和改进的UHPLC-MS分析

Olaf Kunert¹, Fabian Alperth², Elisabeth Pabi²

¹Institute of Pharmaceutical Sciences, Department of Pharmaceutical Chemistry, University of Graz, Universitätsplatz 1, 8010, Graz, Austria.

|December 7, 2023

概括

在Artemisia abrotanum中,主要的甲氧黄被确定为chrysosplenetin,而不是casticin,需要进行结构修订. 先进的NMR和LC-PDA-ESI-MSn方法证实了这一发现.

科学领域:

植物化学植物化学
自然产品化学自然产品化学
有机化学有机化学

背景情况:

阿尔特梅西亚 (Artemisia abrotanum) 是一种以其二次代谢产物而闻名的植物.
之前的研究报告说,在A. abrotanum中,丁素是主要的甲基黄醇.
准确识别植物化合物对于理解它们的特性和应用至关重要.

研究的目的:

从Artemisia abrotanum中分离并从结构上阐明主要的甲氧黄.
为了澄清之前被误认为是素的化合物的结构特征.
开发和应用先进的分析技术,以准确地表征化合物.

主要方法:

从Artemisia abrotanum的上空部分中分离主要的甲氧黄.
核磁共振 (NMR) 谱学,包括使用胺-d5用于增强信号分辨率的2D NMR实验.
液体染色学-光二极管阵列-电子喷射电离-质谱学 (LC-PDA-ESI-MSn) 使用五二 (PFP) 静止相.

主要成果:

主要的甲氧黄被确定为斯普莱尼丁 (1), 与斯丁 (2) 不同的替代模式.
修订了Artemisia abrotanum和Artemisia frigida中化合物的结构分配.
在PFP阶段优化LC-PDA-ESI-MSn提供了有效的分离和独特的碎片化模式 (MS4) 对于chrysosplenetin和casticin.

结论:

克里索斯普莱尼丁是Artemisia abrotanum中主要的甲氧黄的正确标识.
该研究强调了先进的NMR和MS技术对于准确的天然产品结构阐明的重要性.
开发的PFP染色法是有效的分析相关的Artemisia物种的chrysosplenetin和casticin.

关键词:

阿尔特梅西亚 sp. 的种类.阿斯特拉属植物 (Asteraceae) 是一个植物.在卡斯蒂辛,卡斯蒂辛.克里索斯普莱尼丁是什么意思黄类化合物黄类化合物这是NMR的NMR.结构阐明结构阐明在UHPLC-PDA-ESI-MS中使用.

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