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部分或完全丧失诺上腺素会差异地改变海马体CA1中的上下文恐惧和catecholamine释放动态
Leslie R Wilson1,2, Nicholas W Plummer1, Irina Y Evsyukova1
1Neurobiology Laboratory, NIEHS, National Institutes of Health, Department of Health and Human Services, Research Triangle Park, North Carolina.
Biological psychiatry global open science
|December 7, 2023
概括
在上下文恐惧学习过程中,海马体的上腺素 (NE) 和多巴胺 (DA) 释放发生变化. 改变NE合成影响恐惧记忆回忆,时间和严重程度影响结果.
科学领域:
- 神经科学是一个神经科学.
- 行为神经科学 行为神经科学
- 分子神经科学 分子神经科学
背景情况:
- 情境恐惧学习依赖于海马.
- 甲基荷胺 (上腺素和多巴胺) 涉及到记忆,但它们在行为过程中的海马释放动态尚不清楚.
- 需要新的动物模型来研究改变的甲基荷胺合成对释放和学习的影响.
研究的目的:
- 在情境恐惧调节过程中,研究背部海马体中诺拉上腺素 (NE) 和多巴胺 (DA) 释放动态.
- 利用具有改变NE合成的新型小鼠模型和体内传感器来检查catecholamine释放.
- 了解NE合成改变对恐惧记忆回忆的影响.
主要方法:
- 产生了两种新型小鼠模型,具有改变的位置coeruleus-norepinephrine (NE) 合成.
- 使用GRAB_NE和GRAB_DA传感器与体内纤维光度.
- 在上下文恐惧调节期间,在背部海马体CA1中测量NE和DA释放.
主要成果:
- 厌足冲击在背部CA1.1中增加了NE和DA释放.
- 在恐惧记忆回忆期间的结行为与减少NE和DA释放相关.
- 失去NE合成影响了最近的恐惧记忆回忆,而远程回忆受到了产前完全失去NE合成的影响.
结论:
- NE在情境恐惧记忆中起着至关重要的作用.
- 在恐惧回忆过程中,NE和DA表现出不同的时间释放动态.
- 基因型,性别和发育时间与恐惧记忆中的NE信号复杂地相互作用.
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