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Updated: Jul 9, 2025

Application of CRISPR Interference CRISPRi for Gene Silencing in Pathogenic Species of Leptospira
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Application of CRISPR Interference CRISPRi for Gene Silencing in Pathogenic Species of Leptospira

Published on: August 14, 2021

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克里斯普尔-卡斯免疫被LysR型转录调节器PigU抑制

Leah M Smith1,2,3, Hannah G Hampton1, Mariya S Yevstigneyeva1

  • 1Department of Microbiology and Immunology, University of Otago, PO Box 56, Dunedin 9054, New Zealand.

Nucleic acids research
|December 7, 2023
PubMed
概括

猪是细菌CRISPR-Cas免疫力在塞拉蒂亚的主要调节者,控制菌体防御和其他细胞过程. 这一发现优化了CRISPR-Cas调节,以最大限度地降低健身成本和自身免疫力.

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The organization of prokaryotic genes in their genome is notably different from that of eukaryotes. Prokaryotic genes are organized, such that the genes for proteins involved in the same biochemical process or function are located together in groups. This group of genes, along with their regulatory elements, are collectively known as an operon. The functional genes in an operon are transcribed together to give a single strand of mRNA known as polycistronic mRNA.
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Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
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